GC法测定（1R，2R）-（-）-1，2-环己二胺中S，S对映异构体

目的：建立GC法，测定（1R，2R）-（-）-1，2-环己二胺中S,S对映异构体。方法：用三氟乙酸酐对1，2-环己二胺进行柱前衍生化。色谱柱：CHIRALDEX^TM B-DP二丙酰化B-环糊精手性毛细管柱（30m×0．25mm），检测器：FID，进样口及检测器温度：200℃，柱温：175℃，载气：氮气，流速：3．0mL·min^-1，分流比：50：1，进样量：1．0μL。结果：1，2-环己二胺的R，R与S，S对映异构体衍生物达到基线分离；S，S对映异构体定量限和检测限分别为2．2和0．66mg·L^-1，平均回收率为96．2％，RSD为2．1％（n=6）。结论：本法能较为准确、快速、有效地分离测定（1R，2R）-（-）-1，2-环己二胺中S，S对映异构体。

Determination of S,S-enantiomer in (1R,2R)-(-)-1,2-cyclohexanediamine by GC

Objective： To establish a GC method for determination of S, S-enantiomer in （ 1R, 2R） -（ - ） -1, 2-cyclohexanediamine. Methods： The pre-column derivatization of 1, 2-cyclohexanediamine was conducted with TFAA. The GC condition was used as follows： CHIRALDEX^TM B-DP capillary column （ β-cyclodextrin, dipropionyl, 30 m × 0.25 mm）, a FID detector, a sampling/detector temperature of 200 ℃, a column temperature of 175 ℃, N2 as carrier gas, a flow rate of 3.0 mL·min^-1, a split ratio of 50 ： 1 and a sampling volume of 1.0μL. Results： The baseline resolution was achieved between the derivatives of S, S-and R, R-enantiomers of 1, 2-cyclohexanediamine. The quantitative and detective limits were 2.2 and 0. 66 mg·L-1 respectively and the average recovery was 96.2% with RSD of 2.1% （n =6） for the S, S-enantiomer. Conclusion： The method is acurate, rapid and effective for the determination of S, S-enantiomer in （ 1R, 2R） -（ - ） -1,2-cyclohexanediamine.