RP—HPLC法同时测定不同产地红参中6种人参皂苷的含量

目的：建立反相高效液相色谱法同时测定红参中6种人参皂苷Rgl、Re、Rf、Rb1、Re和Ro的含量。方法：采用AlltimaTM．C18柱（250mm×4．6mm，5μm），以0．1％磷酸水溶液-乙腈为流动相进行梯度洗脱，流速为1．0mL·min^-1，检测波长为203nm，柱温为30℃，进样量为10止，外标法计算人参皂苷Rg1、Re、Rf、Rb1、Re和R0的含量。结果：人参皂苷Rg1、Re、Rf、Rb1、Re和R0分别在0．603—6．03μg、0．153～1．53μg、0．157～1．57μg、0．758—7．58μg、0．306～3．06μg和0．768—7．68μg范围内，其质量与峰面积的线性关系良好，r分别为0．9996、0．9996、0．9998、0．9996、0．9996和0．9996；加样回收率（n=6）分别为101．3％、99．2％、98．1％、97．6％、100．7％和99．0％，RSD分别为0．41％、2．03％、1．03％、0．33％、2．31％和1．87％。结论：该法结果准确、简便可行、重复性好，可为红参药材的质量控制提供依据。

Simultaneous Determination of Six Ginsenosides in Red Ginseng of Different Origins by RP-HPLC

Objective： To develop a RP-HPLC method for simultaneous determination of six ginsenosides including Rgl, Re, Rf, Rbl, Re and Ro in red ginseng of different origins. Methods： Analysis was carried out on a AlltimaTM-c18 column （250 mm ×4. 6 mm, 5 μm） eluted with water solution containing 0. 1% phosphoric acid-acetonitrile as the mobile phase in gradient elution at the flow rate of 1.0 mL.min -1. The UV detection wavelength was 203 nm, the temperature of column was 30℃, and the sample injection volume was 10 μL The contents of ginsenosides Rg1, Re, Rf, Rb1, Rc and Ro were calculated by external standard method. Results： The linear ranges of ginsenosides Rgl, Re, Rf, Rbl, Rc and Ro were 0. 603- 6. 03μg （r =0. 999 6）,0. 153-1.53 μg （r =0. 999 6）, 0. 157-1.57μg （r =0. 999 8）, 0. 758-7.58 p,g （r = 0. 999 6） ,0. 306-3.06 ug （ r =0. 999 6） and 0. 768-7.68 ug （r=0. 999 6）. resvectivelv. The average recoveries were 101.3 % （RSD =0.41%）, 99.2 % （RSD =2.03 %）, 98.1% （RSD =1.03%）, 97.6 % （ RSD = 0. 33 %）, 100. 7 % （ RSD = 2. 31%） and 99.0 % （ RSD = 1.87 % ）, resepectively. Conclusion： The HPLC method is accurate, simple and reproducible. It is helpful to control the quality of red ginseng.