| 低分子量地黄多糖对p53基因表达的影响 |
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| 引用本文： | 魏小龙,茹祥斌.低分子量地黄多糖对p53基因表达的影响[J].中国药理学报,1997,18(5):471-474. |
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| 作者姓名： | 魏小龙 茹祥斌 |
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| 作者单位： | 军事医学科学院毒物药物研究所 |
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| 摘 要： | 目的：研究低分子量地黄多糖（ＬＲＰＳ）对ｐ５３基因表达的影响。方法：利用定量ＰＣＲ的方法检测ｐ５３基因表达的水平。结果：ＬＲＰＳ在最适抗肿瘤有效剂量２０和４０ｍｇ·ｋｇ＾－１条件下，Ｌｅｗｉｓ肺癌组织内ｐ５３基因的表达水平分别是１．５２和１．４８，对照组为０．４６，环磷酰胺组为１．６０，ＬＲＰＳ能使Ｌｅｗｉｓ肺癌组织内ｐ５３基因的表达明显增加。结果：ＬＲＰＳ对抗癌基因ｐ５３表达的影响是其抗肿瘤作用
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| 关 键 词： | 地黄 多糖 p53基因 聚合酶链反应 |
Effects of low-molecular-weight Rehmannia glutinosa polysaccharides on p53 gene expression] |
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| X L Wei,X B Ru.Effects of low-molecular-weight Rehmannia glutinosa polysaccharides on p53 gene expression][J].Acta Pharmacologica Sinica,1997,18(5):471-474. |
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| Authors: | X L Wei X B Ru |
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| Institution: | Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing, China. |
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| Abstract: | AIM: To study the effect of low-molecular-weight Rehmannia glutinosa polysaccharides (LRPS) on p53 gene expression. METHODS: The level of p53 gene expression was determined by quantitative polymerase chain reaction and autoradiography. RESULTS: The levels of p53 mRNA in Lewis lung cancer tissue were 0.46, 1.52, 1.48, and 1.60 respectively after saline, LRPS 20 and 40 mg.kg-1, and cyclophosphamide 10 mg.kg-1, which were the effective doses inhibiting the growth of tumor tissue cells in vivo. LRPS markedly increased p53 gene expression. CONCLUSION: LRPS effect on p53 gene expression is one of the mechanisms of antitumor activity. |
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