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噻唑蓝法检测中药复方对人白血病CD34~+CD38~-干细胞群的影响
引用本文:徐瑞荣,王敬毅,崔兴,王琰,胡述博,李丽珍.噻唑蓝法检测中药复方对人白血病CD34~+CD38~-干细胞群的影响[J].中国药物与临床,2008,8(9):696-698.
作者姓名:徐瑞荣  王敬毅  崔兴  王琰  胡述博  李丽珍
作者单位:[1]山东中医药大学附属医院血液科,济南250011; [2]山东大学齐鲁医院免疫室,济南250011;
摘    要:目的运用噻唑蓝(MTT)法检测中药益气养阴方及其拆方后的扶正组方、祛邪组方对人白血病CD34+CD38-干细胞群的影响。方法免疫磁珠分离人白血病CD34+CD38-干细胞群,MTT法检测中药复方对细胞生长的影响。结果益气养阴制剂对白血病干细胞的体外抑制率为(24.390±0.046)%,扶正组抑制率为(9.270±0.052)%,祛邪组为(9.370±0.017)%,益气养阴组与扶正组、祛邪组比较差异均有统计学意义(P<0.05),扶正组与祛邪阻比较差异无统计学意义。结论益气养阴方对白血病干细胞有明显抑制作用。

关 键 词:白血病  噻唑蓝  免疫磁珠

MTT study on the response of human CD34+/CD38-leukemic stem cell to traditional Chinese recipes
XU Rui-rong,WANG Jing-yi,CUI Xing,WANG Yan,HU Shu-bo,LI Li-zhen.MTT study on the response of human CD34+/CD38-leukemic stem cell to traditional Chinese recipes[J].Chinese Remedies & Clinics,2008,8(9):696-698.
Authors:XU Rui-rong  WANG Jing-yi  CUI Xing  WANG Yan  HU Shu-bo  LI Li-zhen
Institution:XU Rui-rong, WANG Jing-yi, CUI Xing, WANG Yah, HU Shu-bo, LI Li-zhen. (Department of Hematology, Shandong Traditional Chinese Medicine University Hospital, Jinan 250011, China)
Abstract:Objective To determine the response of human CD34^+/CD38^- leukemic stem cell (LSCs) to Yiqi Yangyin Fang, a traditional Chinese formula, used as a full package or part of its supportive or aggressive components. Methods Magnetic activated cell sorting (MACS) was used to select CD34^+/CD38^- LSCs. The growth of these cells in response to TCM recipes was determined with MTF assay. Results The growth inhibition was found in (24.390±0.046)% of LSCs exposed to full-package Yiqi Yangyin Fang, compared with (9.270±0.052)% of those exposed to the supportive components and (9.370±0.017)% exposed to aggressive components. CD34^+/CD38^- LSCs were more susceptible to the full-package than to either of its components (P〈0.05) which did not show difference between each other. Conclusion The Yiqi Yangyin formula exhibited remarkable inhibitory effects on leukemic stem cells.
Keywords:Leukemia  MTF assay  MACS
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