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| 异硫氰酸荧光素标记人血清白蛋白 | |
| 引用本文： | 李勇,何俊,但德忠,乔小蓉,李毓琦,郭明秀.异硫氰酸荧光素标记人血清白蛋白[J].华西药学杂志,2001,16(1):25-26. |
| 作者姓名： | 李勇 何俊 但德忠 乔小蓉 李毓琦 郭明秀 |
| 作者单位： | 1. 华西医科大学药学院 2. 四川大学环境科学与工程学院 3. 基础医学院, |
| 摘 要： | 目的：用异硫氰酸荧光素（FTTC）对人血清白（HSA）进行荧光标记。方法：采用直接标记法，并考查不同料比时的荧光素分子与蛋白分子的结合情况，荧光标记物（FTTC－HSA）的荧光光谱及荧光寿命情况，结果：FTTC－HSA的激发波长为500nm，荧光波长为530nm；其固态和液态低温保存时荧光寿命分别为一年和37天。 |
| 关 键 词： | 异硫氰酸荧光素 人血清白蛋白 荧光免疫分析 荧光标 |
| 文章编号： | 1006-0103(2001)01-0025-02 |
| 修稿时间： | 1999年7月1日 |
LABELING OF HUMAN SERUM ALBUMIN WITH FLUORESCEIN ISOTHIOCYANATE |
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| LI Yong,HE Jun,DAN De-zhong,QIAO Xiao-rong,LI Yu-qi,GUO Ming-xiu.LABELING OF HUMAN SERUM ALBUMIN WITH FLUORESCEIN ISOTHIOCYANATE[J].West China Journal of Pharmaceutical Sciences,2001,16(1):25-26. | |
| Authors: | LI Yong HE Jun DAN De-zhong QIAO Xiao-rong LI Yu-qi GUO Ming-xiu |
| Abstract: | OBJECTIVE:To label human serum albumin(HSA).METHODS：In this study,we labeled human serum albumin with fluorescein isothiocyanate(FITC),and then the ratio of FITC to HSA for labeling reaction,the varances of spectra after being labeled and the life of the labeled HSA were optimized.RESULTS：The result showed that the spectra were λex=500 nm，λem=530 nm；the life of the labeled HSA were 37 day and 1 year in liquid and solid,respectively.CONCLUSION：To label HSA with FTIC was successful,and it was applicable to Fluoroimmunoassay. |
| Keywords: | Fluorescein isothiocyanate(FITC) Human serum albumin(HAS) Fluoroimmunoassay(FIA) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |