| 不同提取方法盐全蝎水溶性蛋白含量对比研究 |
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| 引用本文： | 胡晓静,孔成诚,方成武,戴向荣.不同提取方法盐全蝎水溶性蛋白含量对比研究[J].中国医药科学,2011(5):38-39. |
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| 作者姓名： | 胡晓静 孔成诚 方成武 戴向荣 |
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| 作者单位： | 安徽中医学院药学院安徽省现代中药重点实验室;兆科药业(合肥有限公司) |
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| 基金项目： | 安徽省高校自然科学重点项目(KJ2009A113) |
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| 摘 要： | 目的通过对不同提取方法盐全蝎水溶性蛋白含量的比较研究,寻找水溶性蛋白含量较高的提取方法,为全蝎的临床用药提供借鉴。方法对活体东亚钳蝎进行盐制后,分别对水浸泡24h、水煎30、60、90、120min、75%乙醇浸泡24h六组样品浸出液进行粗蛋白、蛋白氮和氨基酸含量的测定。结果水煎60min盐全蝎水提液的粗蛋白、蛋白氮、氨基酸的含量与清水浸泡24h,水煎30min、90min、120min相比具有极显著差异。结论临床应用上采取水煎60min的方法可能更有利于全蝎的药效发挥。
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| 关 键 词： | 盐全蝎 不同提取方法 粗蛋白含量 蛋白氮含量 氨基酸含量 |
Comparison Study of Different Extraction Methods of Salt Buthus Martensii Karsch Comparison Water-soluble Protein |
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| HU Xiaojing,KONG Chengcheng,FANG Chengwu,DAI Xiangrong.Comparison Study of Different Extraction Methods of Salt Buthus Martensii Karsch Comparison Water-soluble Protein[J].China Medicine and Pharmacy,2011(5):38-39. |
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| Authors: | HU Xiaojing KONG Chengcheng FANG Chengwu DAI Xiangrong |
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| Institution: | 1. College of Pharmacy of Anhui College of Traditional Chinese Medicine, Key Laboratory of Modern Chinese Medicine of Anhui,Hefei,230031,China;2. Zhaoke Pharmaceuticals(Hefei) Co,Ltd,Hefei 230031,China |
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| Abstract: | Objective According to the different extraction methods of Buthus martensii Karsch water-soluble protein comparative study,find a best method to play efficacy. Methods After salt making,we chose six methods(water soak 24 hours, boiled 30 minutes,boiled 60 minutes,boiled 90 minutes, boiled 120minutes,75% ethanol soak 24hours) to determinate the solution with crude protein content,protein nitrogen content and amino acids content. Results Compared with water soak 24 hours, boiled 30minutes, boiled 90 minutes,boiled 120minutes,the sample of boiled 60 minutes had significant difference. Conclusion The sample of water boiled 60 minutes may be more beneficial to the clinical efficacy. |
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| Keywords: | Salt Buthus martensii Karsch Different extraction Total protein content Protein nitrogen content Amino acid content |
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