HPLC法测定硝普钠氯化钠注射液中硝普钠的含量

目的：建立HPLC法测定硝普钠氯化钠注射液中硝普钠的含量。方法：Diamonsil ODS C18柱（250nm×4.6nm,5μm）;以0.34%硫酸氢四丁基铵与0.284%无水磷酸氢二钠溶液（用磷酸调pH值至6.0）-甲醇（50∶50）为流动相;流速1.0ml·min-1;检测波长是254nm。结果：硝普钠在9.96～637.44μg·ml-1范围内,（r=0.9998）线性关系良好,平均回收率为99.5%,RSD=0.8%（n=5）。结论：本法简便快捷,准确,可用于硝普钠氯化钠注射液中硝普钠的含量测定。

Determination the Content of Sodium Nitroprusside in Sodium Nitroprusside and Sodium Chloride Injection by HPLC

Objective：To establish amethod for determination the content of sodium nitroprusside in sodium nitroprusside and Sodium Chloride injection by HPLC.Method：Diamonsil ODS C18column（250nm×4.6 nm,5μm）was used,the 0.34% four tetrabutylammonium hydrogen sulfate and 0.284% anhydrous sodium hydrogen phosphate two solution（adjusted with phosphoric acid to pH6）- methanol（50∶50）as the mobile phase,the flow rate was 1.0ml·min-1,the detection wavelength was 254nm.Result：The linear range of sodium nitroprusside was 9.96~637.44μg·ml-1（r=0.9998）,the average recovery was 99.5% with RSD=0.8%（n=5）.Conclusion：The method is fast and accurate.It can be used for determination the content of sodium nitroprusside in sodium nitroprusside and Sodium Chloride injection.