HPLC法同时测定桂枝茯苓胶囊中丹皮酚、芍药苷和苦杏仁苷的含量

目的：建立HPLC法同时测定桂枝茯苓胶囊中丹皮酚、芍药苷和苦杏仁苷的含量。方法：采用HPLC法测定桂枝茯苓胶囊中丹皮酚、芍药苷和苦杏仁苷的含量。采用C18柱，流动相为甲醇∶水（55∶45）洗脱，流速为1.0ml·min-1，检测波长为274nm，柱温为30℃，进样体积为10μL。结果：线性范围分别为0.198～3.166μg（r=0.9998）、0.195～3.128μg（r=0.9996）、0.197～30158（r=0.9997）。平均加样回收率分别为99.81%（RSD=2.48%）、99.69%（RSD=1.47%）、99.79%（RSD=2.12%）。结论：本研究建立的方法简便，结果稳定可靠，可用于桂枝茯苓胶囊的质量控制。

Simultaneous content determination of dan phenol,paeoniflorin and peach kernel in Guizhifuling capsule by HPLC method

Objective To establish an HPLC method for Simultaneous content determination of dan phenol, paeoniflorin and peach kernel in guizhifuling capsule. Methods The content of dan phenol, paeoniflorin and peach kernel in guizhifuling capsule was determinated by HPLC method. The chromatographic condition was C18 column; the mobile phase was methanol-water (55∶45)elution; the flow rate was 1.0ml·min-1; the detection wavelength was 274nm; the column temperature of 30℃; the sample volume was 10μl. Results The linear range were 0.198~3.166μg(r=0.9998), 0.195~3.128μg(r=0.9996), 0.197~30158(r=0.9997)respectively. The average sample recovery rate was 99.81%(RSD=2.48%),99.69% (RSD=1.47%), 99.79%(RSD=2.12%) respectively. Conclusion The established methods is simple by this research, the results is stable and reliable, and can be used for the quality control of guizhifuling capsule.