Relationship between miRNAs polymorphisms and peripheral blood leukocyte DNA telomere length in coke oven workers: A cross-sectional study |
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| Institution: | 1. Department of Occupational Health and Occupational Diseases, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China;2. The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou 450001, Henan, China;3. Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, Henan, China;1. School of Public Health, Southwest Medical University, Luzhou 646000, China;2. Department of Clinical Laboratory, 404 Hospital of Mianyang, Mianyang 621000, China;3. School of Pharmacy, Southwest Medical University, Luzhou 646000, China;4. State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China;5. School of Environmental and Resource, Southwest University of Science and Technology, Mianyang 621010, China;1. PUCRS, Pontifical Catholic University of Rio Grande do Sul, School of Health and Life Sciences, Conservation Physiology Laboratory, Conservation Physiology Laboratory, Porto Alegre, RS, Brazil;2. Pro-Pampa Institute, Pelotas, RS, Brazil;3. UNISINOS, Universidade do Vale do Rio dos Sinos, Centro de Ciências, Programa de Pós-Graduação de Biologia, Laboratório de Ecologia e Conservação de Ecossistemas Aquáticos, São Leopoldo, RS, Brazil;4. FURG, Federal University of Rio Grande, Rectory, Pro-Rectory of Research and Graduate Studies, University of Rio Grande Foundation, Rio Grande, RS, Brazil;1. School of Life Science, College of Environment and Resource, Shanxi University, Taiyuan 030006, China;2. Department of Biochemistry and Molecular Biology, Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan 030001, China |
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| Abstract: | ObjectiveThe purpose of this study was to investigate the factors affecting telomere length (TL) in coke oven workers by analyzing the interaction between miRNAs polymorphisms and coke oven emissions (COEs) exposure.MethodsA total of 544 coke oven workers and 238 healthy controls were recruited. Peripheral blood was collected from the subjects, genomic DNA was extracted, leukocyte TL was detected by real-time quantitative polymerase chain reaction, and fifteen polymorphisms of eight miRNAs were genotyped by flight mass spectrometry.ResultsStatistical analysis showed that the peripheral blood DNA TL in the exposure group was shorter than that in the control group (P < 0.001). Generalized linear model found that COEs-exposure β (95%CI) = -0.427 (?0.556, ?0.299), P < 0.001], genotype CC+CT for miR-612 rs1144925 β (95%CI) = ?0.367 (?0.630, ?0.104), P = 0.006], and the interaction of miR-181B1 rs12039395 TT genotype and COEs-exposure β (95% CI) = 0.564 (0.108, 1.020), P = 0.015] were associated with the shortened TL.ConclusionCOEs-exposure and miR-612 rs1144925 TT could promote telomere shortening in coke oven workers. The interaction of miR-181B1 rs12039395 TT genotype and COEs-exposure could protect telomere. This provides clues for further mechanistic studies between miRNA and telomere damage. |
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| Keywords: | Coke oven emissions Telomere length miRNAs Polymorphisms |
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