Behavioural impairment and oxidative stress by acute exposure of zebrafish to a commercial formulation of tebuconazole |
| |
Institution: | 1. Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, the Netherlands;2. Department of Aquatic Ecology and Water Quality Management, Wageningen University, P.O. Box 47, 6700 AA Wageningen, the Netherlands;3. Thailand Institute of Scientific and Technological Research 35 Moo 3, Tambon Klong five, Klong Laung, PathumThani 12120, Thailand;4. Department of Animal and Plant Sciences, The University of Sheffield, Sheffield S10 2TN, UK;5. Alterra, Wageningen University and Research Centre, P.O. Box 47, 6700 AA Wageningen, the Netherlands |
| |
Abstract: | Tebuconazole is a systemic follicular fungicide known to cause diverse problems in non-target organisms namely associated to the pure active ingredient. As such, the objective of this work was to evaluate developmental changes induced by a tebuconazole commercial formulation to a non-target animal model. Zebrafish embryos at ± 2 h post-fertilization were exposed to tebuconazole wettable powder concentrations (0.05, 0.5 and 5 mg L-1) for 96 h with developmental toxicity assessed throughout the exposure period and biochemical parameters evaluated at the end of the exposure. Behavioural assessment (spatial exploration and response to stimuli) was conducted 24 h after the end of the exposure. While no developmental and physiological alterations were observed, exposure to tebuconazole resulted in an increased generation of reactive oxidative species at the 0.05 and 0.5 mg L-1 concentrations and a decreased GPx activity at the 0.5 mg L-1 concentration suggesting a potential protection mechanism. There was also a change in the avoidance-escape behaviour supporting an anxiolytic effect suggesting possible alterations in the central nervous system development demanding further studies. |
| |
Keywords: | Tebuconazole Zebrafish Embryo development Oxidative stress Behaviour |
本文献已被 ScienceDirect 等数据库收录! |
|