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| 血小板衍生生长因子与CD68在单侧输尿管结扎大鼠肾间质中的表达及意义 | |
| 引用本文： | 刘会卿,左平祥,赵旭兰,赵会颖,陆志修,王静,冯瑾,李荣芬.血小板衍生生长因子与CD68在单侧输尿管结扎大鼠肾间质中的表达及意义[J].河北医药,2009,31(1):15-16. |
| 作者姓名： | 刘会卿 左平祥 赵旭兰 赵会颖 陆志修 王静 冯瑾 李荣芬 |
| 作者单位： | 1. 河北省石家庄市第一医院干部病房,050011 2. 河北省灵寿县医院 3. 河北医科大学第三医院肾内科 |
| 基金项目： | 石家庄市科技支撑计划项目 |
| 摘 要： | 目的研究血小板衍生生长因子（PDGF-BB）及CD68（巨噬细胞标志物）阳性细胞在单侧输尿管结扎（UUO）大鼠肾间质中的表达。方法制作大鼠肾间质纤维化模型,于术后第7、14天用免疫组化方法检测肾间质中PDGF-BB、α-平滑肌肌动蛋白（α-SMA）的表达及CD68阳性细胞（巨噬细胞）浸润,并进行相关性分析。结果与假手术组比较,模型组肾间质中PDGF-BB、和α-SMA的表达水平明显升高（P〈0.05）,CD68阳性细胞浸润明显增多（P〈0.05）,模型组CD68阳性细胞在第14天时较第7天时减少。α-SMA与PDGF-BBT CD68的表达呈正相关（r1=0.92,P〈0.01,r2=0.98,P〈0.01）。结论PDGF-BB和巨噬细胞参与肾间质纤维化早期改变。 |
| 关 键 词： | 肾间质纤维化 免疫组织化学 肽类生长因子 α-平滑肌肌动蛋白 CD68 |
Expression of platelet-derived growth factor-BB and CD68 positive cell in renal interstitial tissues of rats with UUO |
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| Institution: | LIU Huiqing , ZUO Pingxiang , ZHAO Xulan , et al .( The First Hospital of Shijiazhuang , shijiazhuang 050011) |
| Abstract: | Objective To investigate the renal expression of platelet-derived growth factor （PDGF-BB） and CD68 positive cell infiltration in rat with unilateral ureteral obstruction （UUO）. Methods twenty-six famal Sprague Dawley rats were randomized into UUO and Sham-operation（SOR） groups, the kidneys were harversted at 7d, 14d after the operation.immunnohistochemistry was used on the renal tissue for PDGF-BB ,α-SMA and CD68 positive cell which was a maker of maerophage. Results compared with the SOR group , there was a significant increase in PDGF-BB, α-SMA and CD68 expression in the UUO group（ P 〈 0.05）. the expression intensity of α-SMA was correlated with PDGF-BB,CD68 （ r1=0.92, P 〈0.01; r2=0.98, P 〈0.01）.Conclusion PDGF-BB and CD68 plays an important role in the early interstitial fibrosis in kidney. |
| Keywords: | CD68 |
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