黄芪多糖对免疫功能影响的体外实验研究 |
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引用本文: | 刘印华,;李树义,;赵志强,;张庆波.黄芪多糖对免疫功能影响的体外实验研究[J].河北医药,2014(18):2731-2733. |
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作者姓名: | 刘印华 ;李树义 ;赵志强 ;张庆波 |
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作者单位: | [1]河北省南堡盐场医院,河北省唐山市063305; [2]河北联合大学,河北省唐山市063305; |
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基金项目: | 唐山市科学技术研究与发展计划(编号:13130253z) |
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摘 要: | 目的探讨黄芪多糖对小鼠免疫功能的影响,为黄芪多糖的临床应用提供理论依据。方法取小鼠脾细胞,置于96孔细胞培养板中,设黄芪多糖组和空白对照组,WST-1法测细胞的增殖活性;ELISA测细胞因子IL-2、IFN-γ;流式细胞术(FCM)检测细胞表型。结果黄芪多糖组细胞增殖活性显著高于空白对照组(P〈0.01);黄芪多糖组IL-2、IFN-γ的浓度随黄芪多糖浓度的增加而增多,在一定浓度范围内呈剂量依赖关系;黄芪多糖既能刺激B淋巴细胞增殖,也能刺激T淋巴细增殖。结论黄芪多糖可增强免疫功能,它不仅能提高体液免疫功能,而且能提高细胞免疫功能。
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关 键 词: | 黄芪多糖 体液免疫 细胞免疫 干扰素 白细胞介素-2 |
Experimental study on the effect of Astragalus Polysaccharide on immune function in vitro |
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Institution: | LIU Yinhua, LI Shuyi, ZHAO Zhiqiang , et al. ( Hebei Nanpu Saltern Hospital , Hebei , Tangshan 063305, China) |
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Abstract: | Objective To investigate the effect of Astragalus Polysaccharides on the immune function of mice,provide a theoretical basis for its clinical application. Methods The mice spleen cells,which placed in 96-well cell culture plates,were divided into astragalus polysaccharide group and blank control group. The cell proliferation activity was measured by WST-1 method while ELISA method for cell factor IL-2,IFN- γ; flow cytometry( FCM) to detect cell phenotype. Results The proliferation of cell activity of Astragalus polysaccharide group were obvious higher than that of control group( P〈 0. 01); the concentration of IL-2 and IFN- γ in astragalus polysaccharide group increased with the rising concentration of Astragalus polysaccharide,a dose dependent manner in a certain range; astragalus polysaccharides can stimulate the proliferation of B lymphocytes and T lymphocyte. Conclusion Astragalus polysaccharide can enhance immune function. It can not only improve the humoral immune function,but also can increase the cellular immune function. |
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Keywords: | astragalin polysaccharides humoral immune cellular immune IFN-γ IL-2 |
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