| 反相高效液相色谱法测定对乙酰氨基酚片的含量 |
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| 引用本文： | 逯小萌. 反相高效液相色谱法测定对乙酰氨基酚片的含量[J]. 海峡药学, 2016, 0(10): 56-58. DOI: 10.3969/j.issn.1006-3765.2016.10.023 |
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| 作者姓名： | 逯小萌 |
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| 作者单位： | 河南省濮阳市食品药品检验检测中心濮阳457000 |
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| 摘 要： | 目的：建立对乙酰氨基酚片的反相高效液相色谱法。方法色谱柱为Waters-C18（4.6mm ×250mm，5μm），流动相：甲醇-1％冰醋酸溶液（30∶70），流速为1.0mL· min －1，柱温为35℃，检测波长为246nm。结果对乙酰氨基酚进样量在0.0402～1.0055μg范围内与峰面积呈良好线性关系，r＝0.9997，平均回收率为99.43％，RSD＝0.31％（n＝9）。结论该方法准确度、灵敏度及重复性均较好，可作为对乙酰氨基酚片的含量测定。
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| 关 键 词： | 对乙酰氨基酚片 反相高效液相色谱法 含量测定 |
Content Determination of Acetaminophen Tablets by RP-HPLC |
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| Abstract: | OBJECTIVE To establish a RP-HPLC method for the determination of acetaminophen tab-lets.METHODS Column Waters-C18(4.6mm ×250mm,5μm),mobile phase was methanol∶1% acetic acid solu-tion (30∶70),the flow rate was 1.0mL· min-1,the column temperature was 35℃,the detection wavelength was 246nm.RESULTS The sample presented good linear relationship in the range of 0.0402 ~1.0055μg, r =0.9997.The average recovery rate was 99.43%, RSD=0.31%( n=9 ) .CONCLUSION The method was accu-rate,sensitive and reproducible,and can be used as an assay for the determination of the content of acetaminophen tablets. |
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| Keywords: | Tablet paracetamol RP-HPLC Content determination |
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