| 依托泊苷与鲱鱼精DNA相互作用的研究 |
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| 引用本文： | 蔡向阳,江茂生,邱君志,郑新宇,李清禄.依托泊苷与鲱鱼精DNA相互作用的研究[J].海峡药学,2012,24(9):234-236. |
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| 作者姓名： | 蔡向阳 江茂生 邱君志 郑新宇 李清禄 |
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| 作者单位： | 福建农林大学生命科学学院 福州350002 |
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| 基金项目： | 国家自然科学基金(31170025、31070026) |
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| 摘 要： | 目的 研究依托泊苷与DNA的相互作用.方法 采用荧光光谱及紫外光谱法,结合Ⅰˉ效应、离子强度的影响及DNA熔点测定,分析依托泊苷与DNA光谱效应.结果 DNA对依托泊苷的内源荧光有明显的猝灭作用,猝灭过程为静态猝灭；结合常数KA=2.02×10 -4L·mol-1,结合位点数n=0.89.结论 依托泊苷与DNA的结合作用方式可能为沟槽作用.
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| 关 键 词： | 依托泊苷 DNA 荧光光谱 相互作用 |
Studies on the Interaction of etoposide with Herring Sperm DNA |
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| CAI Xiang-yang
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JIANG Mao-sheng
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QIU Jun-zhi
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ZHENG Xin-yu
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LI Qing-li.Studies on the Interaction of etoposide with Herring Sperm DNA[J].Strait Pharmaceutical Journal,2012,24(9):234-236. |
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| Authors: | CAI Xiang-yang JIANG Mao-sheng QIU Jun-zhi ZHENG Xin-yu LI Qing-li |
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| Institution: | (College of life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | OBJECTIVE Investigate the interaction between etoposide and Herring Sperm DNA.METHODS With the fluorescence and UV-Vis spectrophotometry,the quenching of etoposide induced by DNA and the probable mechanism was studied based on I-fluorescence quenchingeffect,influence of ion strength and DNA melting techniques.RESULTS A strong fluorescence quenching of etoposide induced by DNA,and the probable mechanism was a static quenching.The binding constants KA was 2.02×10-4L·mol-1 and the number of binding site was n=0.89.CONCLUSION The intercalation between etoposide and DNA might be groove binding. |
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| Keywords: | Etoposide DNA Fluorescence spectra Interaction |
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