| 超声提取三叶青总黄酮工艺研究 |
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| 引用本文： | 王维华,吴志庄,牛广俊,朱思,李尚智,梁一池.超声提取三叶青总黄酮工艺研究[J].海峡药学,2016(3):45-48. |
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| 作者姓名： | 王维华 吴志庄 牛广俊 朱思 李尚智 梁一池 |
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| 作者单位： | 1. 福建中医药大学药学院 福州350122; 浙江省竹子高效加工重点实验室杭州310000;2. 国家林业局竹子研究开发中心杭州310000;3. 福建中医药大学药学院 福州350122 |
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| 基金项目： | 省发改委项目(闽发改委〔2014〕89号750070019)；浙江省科技厅协同创新项目(2015F50053)；浙江省科技计划项目(2014F10047) |
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| 摘 要： | 目的：为优化三叶青中主要活性成分总黄酮的提取工艺。方法以提取时间、料液比、乙醇浓度和提取温度为试验因素，三叶青中总黄酮提取率为考察指标，在单因素试验基础上，采用响应面设计方法，对三叶青中总黄酮的提取工艺条件进行优化。结果试验确定最佳提取工艺为：20倍量60％乙醇，60℃下超声提取44min。在此工艺条件下，总黄酮提取率为5．55％。结论经验证，采用响应曲面法优选出的工艺稳定，为进一步研究三叶青提供参考依据。
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| 关 键 词： | 三叶青 总黄酮 超声提取 响应曲面法 |
Studies on the Supersonic Extraction Process TotaI FIavonoids from Tet-rastigma Hemsleyanum |
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| Abstract: | OBJECTIVE To optimize extraction technique of total flavonoids from main active components in Tetrastigma Hemsleyanum.METHODS Taking extraction time,solid-liquid ratio,ethanol concentration and extrac-tion temperature as factors,and extraction rate of total flavonoids from Tetrastigma Hemsleyanum as a selection index, based on results of single factor tests,extraction technique of total flavonoids in Tetrastigma Hemsleyanum was opti-mized by the response surface design method.RESULTS The results showed that the optimum conditions were ex-tracting for 44min at 60℃with 60%ethanol concentration,solid-liquid ratio was 1∶20,and extraction rate of total fla-vonoids was 5.55%.CONCLUSION This technique is stable,and provide reference basis for further study of Tet-rastigma Hemsleyanum. |
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| Keywords: | Tetrastigma Hemsleyanum Total flavonoids Supersonic extraction Response surface methodology |
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