正交试验设计法在佛手多糖提取工艺研究中的应用

目的运用正交试验设计法使佛手多糖提取工艺规范化、科学化。方法以佛手多糖含量为指标，提取次数、提取时间、微波功率、料液比为因素，通过单因素试验确定因素水平，利用正交试验设计对微波法提取佛手多糖的T艺进行优化研究。结果微波法提取佛手多糖的最佳工艺条件为，提取时间：8min，微波提取功率：500W，料液比：1：20。结论正交试验设计可以为规范化的中药提取工艺研究提供科学依据。

Application of the orthogonal experimental design in the research of the extracting technics of fructus citrus sarcodactylis polysaccharide

Objective To make the extracting technics of fructus citrus sarcodactylis polysaccharide standardized and scientific through the orthogonal experimental design. Methods The content of fructus citrus sarcodactylis polysaccharide was used as an indicator, the extracting frequency, extracting time, microwave power, and the proportion of liquids were used as the factors, monofactorial experiment was made to determine the level of actor, and the orthogonal experimental design was used to research on the improved technology for the extraction of fructus citrus sarcodactylis polysaccharide through microwave. Results The optimum conditions for the extraction of fructus citrus sarcodactylis polysaccharide through microwave were summarized as follows: the extracting time was 8 minutes, the extracting power of microwave was 500 W, and the proportion of liquids was 1:20. Conclusion The orthogonal experimental design can provide a scientific basis for the study on standardized extracting technics of traditional chinese medicine.