p53基因第72位密码子基因多态性与HPV16相关宫颈癌

目的探讨湖北地区汉族女性人群中p53基因第72位密码子基因多态性与HPV16相关宫颈癌发生的关系。方法随机选取经PCR检测证实HPV16阳性的宫颈病变病例228例。其中病理证实为宫颈浸润癌的156例作为宫颈癌组，宫颈上皮内瘤变或宫颈炎72例为对照组。用直接PCR法检测新鲜组织标本中p53基因第72位密码子的基因型在宫颈癌组与对照组的分布情况，分析p53codon72基因多态性与HPV16阳性宫颈癌之间的相关性。结果所有样本均成功检测出p53codon72基因型。Pro／Pro、Arg／Pro、Arg／Arg在HPV阳性宫颈癌样本中所占比例分别为22．2％、47．2％、30．6％；在对照组中的比例分别为32．7％、53．2％、14．1％。两组总构成比差异有统计学意义（P=0．010）。与其他两种基因型相比，p53codon72Arg／Arg基因型在HPV16阳性宫颈癌样本检出率明显高于在HPV16阳性宫颈上皮内瘤变及宫颈炎组中的检出率（P=0．004，OR=2．680）。结论p53codon72Arg／Arg基因型是湖北地区汉族女性发生HPV16相关宫颈癌的遗传易感因素。

HPV16 infection and p53 codon72 polymorphism in cervical cancer and precursor lesions

Objective To investigate the relationship of p53 codon72 polymorphism with HPV16 infected cervical cancer.Methods A total of 228 samples with cervical disease,who were proved HPV16-positive by PCR testing were randomly selected.Among these,pathological confirmed 156 samples with cervical carcinoma and 72 samples with cervical intraepithelial neoplasiaor or cervicitis were obtained from women undergoing biopsy or surgery.The p53 codon72 polymorphism in the HPV16-positive cervical cancer and precursor lesions were tested by the Direct-PCR method.The relativity of p53 codon72 genotypes and HPV16-positive cervical cancer was analysed.Results The distribution of p53 codon72 genetype Pro/Pro,Arg/Pro,Arg/Arg in the HPV16 infected cervical carcinoma group was 22.2%,47.2%,30.6%,respectively;That in the HPV16 infected CIN-cervicitis group was 32.7%,53.2,14.1%,respectively.The distributions of p53 genotypes were significantly different in the two groups(P=0.010).The frequencies of p53Arg/Arg genetype in HPV16 positive-cervical carcinoma group was statistically different when comparing with the frequencies of p53Pro/Arg and Pro/Pro genetype in the HPV16 positive CIN-cervicitis group (P=0.004,OR=2.680).Conclusion The p53 codon72Arg/Arg genetype is a risk indicator for the tumorigenesis of HPV16 positive-cervical carcinoma in Han people crowd in Hubei areas.