HPLC测定不同产地不同采收期牡丹皮中丹皮酚的含量 |
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引用本文: | 杨晨,方成武,韩燕全,孟楣,杨洁,汪莉.HPLC测定不同产地不同采收期牡丹皮中丹皮酚的含量[J].中国当代医药,2010,17(5):5-7. |
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作者姓名: | 杨晨 方成武 韩燕全 孟楣 杨洁 汪莉 |
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作者单位: | 1. 安徽中医学院,现代中药安徽省重点实验室,安徽合肥,230031 2. 安徽省中医院,国家三级中药药剂实验室,安徽合肥,230031 |
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基金项目: | 美国许氏基金会资助项目;项目编号XSYJ-2007CN-1 |
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摘 要: | 目的:用高效液相色谱法测定不同产地、不同采收期的牡丹皮中丹皮酚含量,并初步比较不同产地、不同采收期丹皮的质量差异。方法:采用高效液相色谱法测定丹皮酚的含量,色谱柱:Aglent TC—C18柱(4.6mm×250.0mm,5μm),流动相:甲醇:水(60:40),检测波长:274nm,柱温:25℃。结果:在选定色谱条件下线性范围良好,丹皮样品加样回收率为97.25%.RSD值为1.82%。结论:安徽凤凰山产区牡丹皮中丹皮酚的含量高于其他产区,其中以3年生10月含量为最高。
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关 键 词: | 牡丹皮 丹皮酚 含量测定 高效液相色谱 |
Detection the content of paeonol in tree peony root-bark by HPLC |
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Authors: | YANG Chen FANG Chengwu HAN Yanquan MENG Mei YANG Jie WANG Li |
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Institution: | 1.College of Traditional Chinese Medicine in Anhui, Hefei 230031, China; 2.Traditional Chinese Medical Hospital in Anhui, Hefei 230031, China) |
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Abstract: | Objective: To detect different areas of production and different time limit of growth on paeonol in tree peony root-bark by HPLC, and compared with them. Methods: HPLC was carried out under the conditions as follows: Aglent TC-C18 (4.6 mm×250.0 mm, 5 μm) at 25℃ using CH3OH:H20(60:40)as mobile phase, detection wavelength was at 274 nm. Results: The good linear relationship was obtained under the optimum conditions. The recoveries of the standards added Peony tree bark phenol 97.25%, respectively, while the relative standard deviations were 1.82%(n=6). Conclusion: The content of paeonol in Fenghuang Mountain is higher than other areas and the October of three years is the highest. |
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Keywords: | Tree peony root-bark Paeonol Content detection HPLC |
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