| RP-HPLC法测定牛黄上清胶囊中黄芩苷的含量 |
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| 引用本文： | 黄花红,杨燕飞.RP-HPLC法测定牛黄上清胶囊中黄芩苷的含量[J].现代医药卫生,2010,26(7):1018-1019. |
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| 作者姓名： | 黄花红 杨燕飞 |
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| 作者单位： | 1. 淮安市妇幼保健院,江苏,淮安,223001
2. 镇江市药品检验所,江苏,镇江,212003 |
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| 摘 要： | 目的:探讨RP-HPLC法测定牛黄上清胶囊中黄芩苷的含量.方法:采用十八烷基硅烷键合硅胶柱;流动相:甲醇-水-磷酸(47:53:0.2)为流动相,检测波长280nm;流速为1.0 ml/min.结果:黄芩苷线性范围19.935～99.674μg/mL.平均回收率为98.79%,RSD=1.21%.结论:方法简便、快速、结果准确、重现性好,可用于牛黄上清胶囊中黄芩苷的含量测定.
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| 关 键 词： | 牛黄上清胶囊 黄芩苷 HPLC |
Determination of baicalin in Niuhuang Shangqing Capsules by RP-HPLC |
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| HUANG Hua-hong
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YANG Yan-fei.Determination of baicalin in Niuhuang Shangqing Capsules by RP-HPLC[J].Modern Medicine Health,2010,26(7):1018-1019. |
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| Authors: | HUANG Hua-hong YANG Yan-fei |
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| Institution: | HUANG Hua-hong1,YANG Yan-fei2(1.Huai\'an Hospital for Maternity , Child Health Care,Huai\'an Jiangsu 223001,China,2.Zhenjiang Institute for Drug Control,Zhenjiang,Jiangsu 212003,China) |
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| Abstract: | Objective：To establish a RP-HPLC method for the determination of baicalin in Niuhuang Shangqing Capsules.Methods：18 alkyl silane bonded silica gel column was used.The mobile phase was methanol - water - phosphoric acid （47：53：0.2）,the detection wavelength was 280nm;the flow rate was 1.0 mL/min.Results：The linear range of baicalin was 19.935-99.674ug/mL.The average recovery was 98.79%, RSD=1.21.Conclusion ：The method is convenient,fast, reproducible and accurate,which can be used for the content determination of buicalin in Niuhuang Shangqing Capsules. |
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| Keywords: | Niuhuang Shangqing Capsules Baicalin HPLC |
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