双氢青蒿素诱导肺癌细胞凋亡的实验研究

双氢青蒿素是半合成的青蒿素衍生物,近年来研究表明青蒿素类药物具有明显抑制肿瘤生长的作用。目的:探讨双氢青蒿素对肺癌细胞生长抑制作用及其机制。方法:以体外培养的SPC-A-1细胞株为研究对象,采用MTT法测定二氢青蒿素对SPC-A-1细胞的增殖影响,采用TUNNEL染色、DNAladder及流式细胞术检测双氢青蒿素诱导SPC-A-1细胞凋亡。结果:DHA对SPC-A-1细胞的抑制增值作用呈浓度依赖性和时间依赖性特点,双氢青蒿素抑制SPC-A-1细胞增殖作用主要由于其诱导肺癌细胞凋亡所致。

Effect of Dihydroartemisinin on Proliferation of Human lung Cancer Cell Line SPC-A-1

Objective：Dihydroartemisinin（DHA）,a semi-synthetic derivative of artemisinin isolated from the traditional Chinese herb Artemisia annua,is an effective novel antimalarial drug.Recent studies suggest that it also has anticancer effects.Purpose The present study was designed to investigate the effects and mechanisms of DHA on cultured human lung cancer cells（SPC-A-1 cells）.Methods：The cell viability was measured by MTT assay.The apoptosis induction was examined by DNA ladder,tunnel staining.The apoptotic rate was measured by flow cytometry.Results：After treatment with DHA,a decrease in the viability of SPC-A-1 cells and apoptosis were observed.Conclusions：These results demonstrated that DHA can inhibit the lung cancer cells growth and this effect was mainly due to apoptotic induction of lung cancer cells.