桔梗汤总皂苷对慢性阻塞性肺病大鼠IKK/NF-KB/MUC5AC通路的影响

目的： 考察桔梗汤总皂苷对慢性阻塞性肺病大鼠IKK/NF-KB/MUC5AC通路的影响，探讨桔梗汤总皂苷治疗COPD可能的分子机制。方法： 建立COPD大鼠模型，将60只SD大鼠分为空白组、模型组、阳性对照组及不同剂量的桔梗汤总皂苷治疗组，经过3周治疗后，对比分析大鼠肺功能变化、肺灌洗液中IL-6、IL-8、IL-1β的含量、血清中S100A8、S100A9、LCN1、LCN2的含量、肺组织病理切片及IKK2、p-IKBα、NF-KB、MUC5AC蛋白表达量。结果： 各个剂量的桔梗汤总皂苷均可显著改善COPD模型大鼠的肺功能受损，降低COPD大鼠肺灌洗液中IL-6、IL-8、IL-1β的含量，降低血清中S100A8、S100A9、LCN1、LCN2的含量，且均呈现良好的量效关系；另外高剂量的桔梗汤总皂苷在上述所有指标的检测中，与空白组大鼠无显著性差异；Western blot实验结果表明，桔梗汤总皂苷可显著降低COPD大鼠肺组织中IKK2、p-IKBα、NF-KB、MUC5AC蛋白表达，差异具有统计学意义。结论： 桔梗汤总皂苷可显著改善COPD大鼠的肺功能，其机制是通过抑制高度活化的IKK/NF-KB/MUC5AC通路实现的。

Effect of totalsaponins of Radix Platycodonis Decoction on IKK/NF-KB/MUC5AC pathway in rats with chronic obstructive pulmonary disease

OBJECTIVE To explore the effect of total saponins of Platycodon grandiflorum decoction (SPG) on IKK/NF-KB/MUC5AC pathway in rats with chronic obstructive pulmonary disease (COPD) and elucidate the possible molecular therapeutic mechanism of SPG for COPD.METHODS After establishing a model of COPD, 60 Sprague-Dawley rats were divided into the groups of blank, model, positive control and different therapeutic doses of SPG.After 3-week treatment, the following test parameters were compared:lung function changes, contents of interleukin-6 (IL-6), interleukin-8 (IL-8) and interleukin-1β (IL-1β) in lung lavage fluid, serum contents of S100A8, S100A9, LCN1, LCN2, pathological sections of lung tissue and protein expressions of IKK2, p-IKBα, NF-KB and MUC5AC in lung tissue.RESULTS All doses of SPG could significantly improve lung function impairment, lower the contents of IL-6, IL-8 and IL-1β in lung lavage fluid, reduce the serum contents of S100A8, S100A9, LCN1, LCN2 and all demonstrated an excellent dose-effect relationship.Furthermore, high-dose SPG differed not significantly from those in blank group regarding all the above parameters.Western blot indicated that SPG could significantly lower the protein expressions of IKK2, p-IKBα, NF-KB and MUC5AC in lung tissue.And the differences were statistically significant.CONCLUSION SPG can significantly improve lung function of COPD rats.The mechanism is achieved by a suppression of highly activated IKK/NF-KB/MUC5AC pathway.