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蝮蛇抗栓酶对兔血管损伤后血管平滑肌细胞C—myc原癌基因表达的影响
引用本文:张川,艾淑智,张静,季军.蝮蛇抗栓酶对兔血管损伤后血管平滑肌细胞C—myc原癌基因表达的影响[J].中国医院药学杂志,2001,21(3):131-133.
作者姓名:张川  艾淑智  张静  季军
作者单位:深圳市孙逸仙心血管医院,广东 深圳 518001
摘    要:目的:探讨蜊蛇抗栓酶抑制血管再狭窄的作用机制。对临床应用提供理论依据。为再狭窄的防治寻找治疗药物。方法:建立兔髂动脉再狭窄模型。用自行设计、全盛的C-myccDNA探针和原位杂交技术观察血管内膜C-mycmRNA的表达与内膜血管平滑细胞(cscularsmooth muscle cells,vSMCs)增殖和内膜形成的关系。结果:C-mycmRNA表达于动脉受损后1周即达高峰并分布于增生的内膜全层,平均阳性细胞个数为29.23.min^-2,蜊蛇抗栓酶1周时对C-mycmRNA的表达有明显的抑制作用,平均阳性细胞个数为10.73.mm^-2。结论:抑制C-myc基因的表达可能减少或阻止内明显病变的形成,有利于血管再狭窄的防治,研究显示蜊蛇抗栓酶对防治再狭窄有效。

关 键 词:蝮蛇抗栓酶  血管再狭窄  C-myc原癌基因  血管损伤  血管平滑肌细胞  
文章编号:1001-5213(2001)03-0131-03
修稿时间:2000年1月10日

The effect of ahylysantinfarctase on the expression of C-myc oncogene by rabbit vascular smooth muscle cells after denudation
ZHANG Chuan,AI Shu-Zhi,ZHANG Jing,et al.The effect of ahylysantinfarctase on the expression of C-myc oncogene by rabbit vascular smooth muscle cells after denudation[J].Chinese Journal of Hospital Pharmacy,2001,21(3):131-133.
Authors:ZHANG Chuan  AI Shu-Zhi  ZHANG Jing  
Abstract:OBJECTIVE:To elucidate the mechanisms of ahylysantinfarctase toinhibit the vascular restinosis for its clinical application.METHODS:A restinotic model was constructed by injury of rabbit iliac arteries with balloon catheters and a probe designed for rabbit C-myc mRNA was used to detect the expression of it by intimal vSMCs on the vascular cross sections using an in situ hybridization technique at the indicated times. Ahylysantinfarctase was given muscularly (0.01 u.kg-1.d-1) and the relation of this gene expression to the proliferation of vSMCs and vascular intimal formation were estimated.RESULTS:C-myc expression reached its peak level and its positive cells were even distributed in vascular intimas one week after denudation (29.23 positive cells/mm2) and ahylysantinfarctase could significantly inhibit the expression of C-myc oncogene (10.73 positive cellsper mm2) as we calculated the average number of C-myc mRNA positive cells per millimetre intimal area at×400 magnification.CONCLUSIONS:The inhibition of the expression of C-myc oncogene could reduce or stop the formation of vascular intimal lesions and the use of this drug could benefit to managment of restionosis after a successful percutaneous transluminal coronary angioplasty.
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