| 双(7)-他克林对慢性脑缺血老龄大鼠海马神经损伤及认知障碍的改善作用 |
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| 引用本文： | 舒细记,柳威,张磊,杨荣,杨友华,沙佩林,易卉玲,李长雷,艾永循.双(7)-他克林对慢性脑缺血老龄大鼠海马神经损伤及认知障碍的改善作用[J].中国医院药学杂志,2011(21). |
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| 作者姓名： | 舒细记 柳威 张磊 杨荣 杨友华 沙佩林 易卉玲 李长雷 艾永循 |
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| 作者单位： | 江汉大学医学院基础医学部;江汉大学医学院医学实验教学中心; |
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| 基金项目： | 武汉市科技计划项目(编号:201051099415-14) |
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| 摘 要： | 目的:探讨双(7)-他克林(bis(7-)tacrine,B7T)]对慢性脑缺血老龄大鼠认知障碍及海马锥体细胞的保护作用,并分析其促神经细胞增生与认知功能改善的相关性。方法:采用安全随机法将45只12月龄雄性SD大鼠分为缺血组、对照组和干预组,每组15只。采用水迷宫实验评估大鼠学习记忆等认知功能,免疫组化及荧光染色后对海马CA1区锥体细胞、凋亡细胞进行计数。结果:行为学水迷宫实验显示:缺血组整体逃逸时间明显长于对照组和干预组(P<0.01);缺血组停留于平台所在象限的时间明显少于对照组和干预组(P<0.05);缺血组跨过平台区域的次数显著少于对照组(P<0.01)和干预组(P<0.05)。免疫组化染色显示:缺血组海马CA1区NeuN阳性细胞明显少于对照组(P<0.01)和干预组(P<0.05)。荧光染色显示:缺血组凋亡细胞明显多于对照组(P<0.01)和干预组(P<0.05)。认知改善与锥体细胞增生、凋亡抑制的相关性分析:缺血组、对照组及干预组海马CA1区锥体细胞数量、凋亡细胞数量变化分别与空间记忆功能改善呈正相关、负相关。结论:慢性脑缺血后老龄大鼠学习记忆能力受损,B7T可通过促进海马锥体细胞增生、抑制细...
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| 关 键 词： | 慢性脑缺血 B7T 认知 凋亡 老龄大鼠 |
Protective effects of bis(7)-tacrine on cognitive impairment and neuropathology in chronic cerebral ischemic aged rats |
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| SHU Xi-ji,LIU Wei,ZHANG Lei,YANG Rong,YANG You-hua,SHA Pei-lin,YI Hui-ling,LI Chang-lei,AI Yong-xun.Protective effects of bis(7)-tacrine on cognitive impairment and neuropathology in chronic cerebral ischemic aged rats[J].Chinese Journal of Hospital Pharmacy,2011(21). |
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| Authors: | SHU Xi-ji LIU Wei ZHANG Lei YANG Rong YANG You-hua SHA Pei-lin YI Hui-ling LI Chang-lei AI Yong-xun |
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| Institution: | SHU Xi-ji1,LIU Wei1,ZHANG Lei1,YANG Rong1,YANG You-hua1,SHA Pei-lin1,YI Hui-ling2,LI Chang-lei2,AI Yong-xun1(1.Department of Basic Medical Sciences,2.Medical Experimental Teaching Center,Medical College of Jianghan University,Hubei Wuhan 430056,China) |
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| Abstract: | OBJECTIVE To study the protective effects of bis(7)-tacrine(B7T) on cognitive impairment and neuropathology in chronic cerebral ischemic aged rats,and analyze the correlation between improvement of cognition and enhancement of neuro-hyperplasia.METHODS 45 male twelve-month SD rats were randomly divided into ischemic group,control group and B7T-treated group,15 rats in each group.Spatial learning and memory was accessed by Morris Water Maze,immuno-histochemistry and immuno-fluorescence were employed to detec... |
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| Keywords: | chronic cerebral ischemia bis(7)-tacrine cognition apoptosis aged rat |
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