|
|||||
|
|
| 反相高效液相色谱法制备纯辣椒素的研究 | |
| 引用本文： | 吴波,谭文界,付玉梅.反相高效液相色谱法制备纯辣椒素的研究[J].中南药学,2006,4(3):178-179. |
| 作者姓名： | 吴波 谭文界 付玉梅 |
| 作者单位： | 1. 广州医学院药物研究中心,广州,510182 2. 湖北中医学院植化教研室,武汉,430061 3. 广州医学院荔湾医院,广州,510070 |
| 基金项目： | 广东省中医药管理局科研项目 |
| 摘 要： | 目的 从辣椒中制备纯辣椒素。方法从辣椒中提取、分离和纯化出辣椒辣素，然后利用RP-HPLC制备纯辣椒素，并进行HPLC和MS分析。结果从辣椒中分离到一种白色片状结晶，其MS谱显示有2个分子离子峰（m/e305和m/e307）；HPLC分析出现2个色谱峰，进一步用HPLC分离该结晶制得的组分其HPLC和MS均显示为单一化合物。结论根据最终化合物的MS谱与标准辣椒素质谱对比，用此方法制得的辣椒素是单体。 |
| 关 键 词： | 辣椒素 制备 分析 |
| 文章编号： | 1672-2981（2006）03-0178-02 |
| 收稿时间： | 2005-11-08 |
| 修稿时间： | 2005-11-082006-02-17 |
Preparation of pure capsaicin with RP-HPLC method |
|
| Wu Bo,Tan Wen-jie,Fu Yu-mei.Preparation of pure capsaicin with RP-HPLC method[J].Central South Pharmacy,2006,4(3):178-179. | |
| Authors: | Wu Bo Tan Wen-jie Fu Yu-mei |
| Institution: | 1. Research Center of Medicine, Guangzhou Medical College, Guangzhou 510182; 2. Department of Phytochemistry, Hubei College of Traditional Chinese Medicine, Wuhan 430061; 3. Liwan Hospital, Cuangzhou Medical College, Guangzhou 510070 |
| Abstract: | OBJECTIVE To obtain pure capsaicin from the fruit of capsicum. METHODS Capsaicinoid was ex tracted, isolated and purified from the fruit of capsicum. Capsaicin was isolated by RP-HPLC, and analyzed by GCMS and HPLC. RESULTS A white crystal was isolated from the fruit of capsicum. Its mass spectrum indicated two molecular ion peaks at m/e 305 and m/e 307. Mass spectrum and HPLC spectrum of the sample showed pure compound. CONCLUSIONS The pure compound was identified as capsaicin according to the GC-MS data. |
| Keywords: | capsaicin isolation analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |