| 不同虫龄米缟螟所产三叶虫茶HPLC指纹图谱的研究 |
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| 引用本文： | 肖美凤,文礼章,许光明,彭新君. 不同虫龄米缟螟所产三叶虫茶HPLC指纹图谱的研究[J]. 中南药学, 2009, 7(5): 374-377 |
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| 作者姓名： | 肖美凤 文礼章 许光明 彭新君 |
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| 作者单位： | 1. 湖南中医药大学药学院中药学重点学科,长沙,410208
2. 湖南农业大学生物安全科学技术学院,长沙,410128 |
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| 基金项目： | 湖南省卫生厅资助项目 |
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| 摘 要： | 目的建立不同虫龄米缟螟幼虫所产三叶虫茶的HPLC指纹图谱。方法采用HPLC法，色谱条件：Sinochrom ODS-BP柱（250mm×4．6mm，5μm）；流动相：乙腈-1％醋酸水溶液，梯度洗脱；流速为1．0mL·min^-1；检测波长为285nm；柱温为40℃。结果通过检测6批次三叶虫茶，建立其HPLC指纹图谱，共标定了26个共有指纹峰，并且比较了其中的白杨素苷和白杨素的含量。结论所用方法稳定、重复性好，可用于三叶虫茶的质量控制。
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| 关 键 词： | 三叶虫茶 白杨素苷 白杨素 指纹图谱 HPLC法 |
HPLC fingerprint of Sanye Chongcha produced by different ages of Aglossa Dimidiata |
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| XIAO Mei-feng,WEN Li-zhang,XU Guang-ming,PENG Xin-jun. HPLC fingerprint of Sanye Chongcha produced by different ages of Aglossa Dimidiata[J]. Central South Pharmacy, 2009, 7(5): 374-377 |
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| Authors: | XIAO Mei-feng WEN Li-zhang XU Guang-ming PENG Xin-jun |
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| Affiliation: | 1. Key Discipline of Chinese Medicinal Herbs, School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha , 410208; 2. College of Bio-Safety Science and Technology, Hunan Agricultural University , Changsha , 410128) |
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| Abstract: | Objective To develop an HPLC fingerprint of Sanye Chongcha produced by different ages of Aglossa Dimidiata. Methods HPLC was used. Chromatogram column was Sinoehrom ODS-BP （250mm×4.6mm, 5 btm）; the mobile phase consisted of acetonitrile-water containing 1% acetic acid with gradient elution; the flow rate was 1.0 mL· min^-1. The detection wavelengh was 285 nm and column temperature was 40℃. Results After detecting 6 different batches of Sanye Chongcha, an HPLC fingerprint of Chongeha was developed with 26 common peaks simultaneously and the content of chrysin-glucoside and chrysin was compared. Conclusion The method is stable and repeatable, which can be used for the quality control of Sanye Chongcha. |
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| Keywords: | Sanye Chongcha ehrysin-7-glueoside ehrysin fingerprint HPLC |
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