|
|||||
|
|
| 大孔树脂分离纯化小果博落回果实中普托品类总生物碱的研究 | |
| 引用本文： | 黄金红,曾建国,郭宇鸽,谈满良,段晓鹏.大孔树脂分离纯化小果博落回果实中普托品类总生物碱的研究[J].中南药学,2011,9(12):897-901. |
| 作者姓名： | 黄金红 曾建国 郭宇鸽 谈满良 段晓鹏 |
| 作者单位： | 1. 湖南中医药大学,长沙410208;湖南省中药提取工程研究中心,长沙410331 2. 湖南农业大学,长沙410128;湖南省中药提取工程研究中心,长沙410331 3. 湖南省中药提取工程研究中心,长沙,410331 4. 湖南中医药大学,长沙,410208 |
| 摘 要： | 目的 研究不同型号大孔吸附树脂纯化小果博落回果实中的普托品类总生物碱(以原阿片碱和别隐品碱计)的工艺条件及参数.方法 以静态饱和吸附量、静态洗脱率为考察指标,比较了5种大孔树脂,并对AB-8树脂的吸附量、吸附率、洗脱率进行了考察,以普托品类总生物碱转移率和纯度为指标对树脂吸附工艺条件进行了筛选.结果 所比较的5种树脂中,AB-8型树脂具有最佳吸附及洗脱参数,其最佳工艺；上柱液浓度为0.258 mg·mL-1,上柱液pH=8,上柱流速为1mL· min-1,90％乙醇洗脱,洗脱剂用量为3BV,对小果博落回普托品生物碱的动态吸附率和洗脱率均＞90％,洗脱物中普托品类总生物碱含量＞90％.结论 AB-8树脂适合于小果博落回果实中普托品类生物碱的分离与纯化. |
| 关 键 词： | 大孔吸附树脂 小果博落回 分离纯化 原阿片碱 别隐品碱 |
Separation and purification of protopine alkaloids from Macleaya microcarpa(Maxim.) Fedde fruit by macroreticular resin |
|
| HUANG Jin-hong , ZENG Jian-guo , GUO Yu-ge , TAN Man-liang , DUAN Xiao-peng.Separation and purification of protopine alkaloids from Macleaya microcarpa(Maxim.) Fedde fruit by macroreticular resin[J].Central South Pharmacy,2011,9(12):897-901. | |
| Authors: | HUANG Jin-hong ZENG Jian-guo GUO Yu-ge TAN Man-liang DUAN Xiao-peng |
| Institution: | HUANG Jin-hong1,3,ZENG Jian-guo2,GUO Yu-ge1,TAN Man-liang3,DUAN Xiao-peng1(1.Hunan University of Traditional Chinese Medicine,Changsha 410208,2.Hunan Agricultural University,Changsha 410128,3.Hunan Engineering Research Center of Botanical Extract,Changsha 410331) |
| Abstract: | Objective To investigate the technology for adsorbing and separating protopine alkaloidsfrom Macleaya microcarpa(Maxim.) Fedde fruit with different types of macroporous resin(MR). Methods The static capacity adsorption and static elution ratio of 5 types of MR were studied and compared to find the optimum one.The absorbance and adsorption rate of AB-8 and the elution were compared to obtain the optimum technological parameters. Results The AB-8 type of MR possessed optimum adsorption and elution parameters.... |
| Keywords: | macroporous resin Macleaya microcarpa(Maxim ) Fedde separation and purification protopine allocryptopine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |