高效液相色谱法测定人血浆中扎托布洛芬的浓度

目的建立高效液相色谱法测定人血浆中扎托布洛芬浓度的方法。方法采用沉淀法,将待测血浆样品经乙腈沉淀后,以双氯芬酸钠为内标,用高效液相色谱法进行检测。采用Platisil ODS C18色谱柱（250 mm×4.6 mm,5μm）,流动相：0.05 mol.L^-1磷酸二氢钠（三乙胺调pH=5.1）-乙腈（58∶42）;流速：1.2 mL.min^-1;检测波长：330 nm。结果本方法扎托布洛芬标准曲线范围为0.05～30.00μg.mL^-1（n=7）,r〉0.99,最低定量浓度0.05μg.mL^-1。日内和日间RSD均〈7.9%。提取回收率为74.13%～78.64%,内标提取回收率为80.32%～86.13%。结论该方法简便、快速,灵敏度高,专属性强,适用于人血浆中扎托布洛芬浓度的测定。

Determination of zaltoprofen in human plasma by HPLC

Objective To establish a high performance liquid chromatography （HPLC） method to determine zaltoprofen in human plasma. Methods An internal standard （diclofenac sodium） and sedimentation process with acetonitrile were used for the plasma. The compounds were separated on a Platisil ODS Ct8 column （250 mm ×4.6 mm, 5 μm）, 0.05 mol. L^ - 1 sodium dihydrogen phosphate （pH ： 5.1 by triethanolamine）- acetonitrile （58 ： 42） as the mobile phase at 1.2 mL .min - 1. The detection wavelength was 330 nm. Results The assay had the calibration range from 0.05 to 30.00μg . mL ^-1 （n ： 7）, r 0.99, and the lower limit of quantification was 0.05 μg . mL^-1 for zaltoprofen. The intra-day and inter-day precisions （RSD） were lower than 7.9%. The extraction recovery of zaltoprofen was 74.13 % - 78.64 %. The extraction recovery of the internal standard was 80.32% - 86.13%. Conclusion The method is simple, accurate, sensitive and specific for the determination of zaltoprofen in human plasma.