高效液相色谱法测定广藿香中毛蕊花糖苷的含量

目的建立广藿香中毛蕊花糖苷含量的测定方法。方法采用HPLC法,色谱条件为Kromasil C18色谱柱(4.6 mm×250 mm,5μm);流动相为甲醇-0.1%醋酸水溶液(40:60);流速为1 m L·min-1;检测波长334 nm。结果毛蕊花糖苷进样量在0.900¹8.00μg与峰面积呈良好线性关系,r=0.999 9,低、中、高浓度加样平均回收率在99.9%18.00μg与峰面积呈良好线性关系,r=0.999 9,低、中、高浓度加样平均回收率在99.9%¹04.1%(n=3),RSD<1.5%。结论该方法简单可行,重复性好,可作为广藿香药材质量标准控制的方法。

HPLC determination of verbascoside in Pogostemon cablin

Objective To establish an HPLC method to determine the content of verbascoside in Pogostemon cablin. Methods The conditions of chromatography was as follows： Kromasil C18（250 mm×4.6 mm, 5 μm） with a mobile phase of methanol-0.1% acetic acid（40 ：60）. The flow rate was 1.0 m L·min^- 1. The detection wavelength was 334 nm. Rseults There was a good linearity between the verbascoside content and the peak area at 0.900- 18.00 μg, r= 0.999 9, the average recovery of the low, middle and high concentration was at 99.9%- 104.1%（n = 3）, RSDwas less than 1.5%. Conclusion The method is simple, feasible and reproducible, which can be used to control the quality of Pogostemon cablin.