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| 蟾蜍毒素对S180荷瘤小鼠肉瘤细胞Mg~(2+)-ATP酶、葡萄糖-6-磷酸酶活性的影响 | |
| 引用本文： | 赵明芳,王哲,魏龙春,侯科佐,刘云鹏.蟾蜍毒素对S180荷瘤小鼠肉瘤细胞Mg~(2+)-ATP酶、葡萄糖-6-磷酸酶活性的影响[J].实用药物与临床,2009,12(6):385-387. |
| 作者姓名： | 赵明芳 王哲 魏龙春 侯科佐 刘云鹏 |
| 作者单位： | 1. 中国医科大学附属第一医院肿瘸内科,沈阳,110001 2. 辽宁中医药大学病理科,沈阳,110032 3. 辽河油田中心医院麻醉科,盘锦,124010 |
| 基金项目： | 辽宁省教育厅青年基金资助项目 |
| 摘 要： | 目的探讨醇溶性蟾蜍毒素对S180荷瘤小鼠肉瘤细胞Mg2+-ATP酶(Mg2+-ATPase)、葡萄糖-6-磷酸酶(G-6-Pase)活性的影响。方法乙醇溶解蟾蜍分泌物原浆,采用减压蒸馏等方法,进一步部分分离纯化,获得醇溶性的蟾蜍毒素混合物(Ethanol extract of toad venom,简称EET),将其作用于S180荷瘤小鼠模型,计算肿瘤抑制率,应用酶化学染色法观察S180肉瘤细胞酶活性的变化。结果蟾蜍毒素组和氟脲嘧啶组的肿瘤抑制率分别为35.6%和36.9%(P<0.05),细胞质膜标志酶Mg2+-ATPase、内质网标志酶G-6-Pase反应颗粒变小,数量减少,密度变低,提示酶活性明显下降。结论蟾蜍毒素可降低S180荷瘤小鼠肉瘤细胞质膜标志酶Mg2+-AT-Pase及内质网标志酶G-6-Pase的活性,抑制肿瘤细胞的生长。 |
| 关 键 词： | 肿瘤 蟾蜍毒素 S180肉瘤 Mg2+-ATPase 葡萄糖-6-磷酸酶 |
Effect of ethanol extract of toad venom on the activity of Mg2+-ATPase and G-6-Pase in bearing-S180 tumors mice |
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| ZHAO Ming-fang,WANG Zhe,WEI Long-chun,HOU Ke-zuo,LIU Yun-peng.Effect of ethanol extract of toad venom on the activity of Mg2+-ATPase and G-6-Pase in bearing-S180 tumors mice[J].Practical Pharmacy and Clinical Remedies,2009,12(6):385-387. | |
| Authors: | ZHAO Ming-fang WANG Zhe WEI Long-chun HOU Ke-zuo LIU Yun-peng |
| Institution: | ZHAO Ming-fang1,WANG Zhe2,WEI Long-chun3,HOU Ke-zuo1,LIU Yun-peng1(1.Department of Medical Oncology,The First Affiliated Hospital of China Medical University,Shenyang 110001,China,2.Department of Pathology,Liaoning College of TCM,Shenyang 110032,3.Department of Anesthesiology,The Central Hospital of Liaohe Oil Field,Panjin 124010,China) |
| Abstract: | Objective To observe the effect of ethanol extract of toad venom on the activity of Mg2+-ATPase and G-6-Pase in bearing-S180 tumors mice.Methods The models of bearing solid tumors were made by incubated S180 cell suspension to back.All 60 mice were distributed into 3 groups and injected 0.5mg/kg ethanol extract of toad venom or fluorouracil to abdominal cavity,respectively.The mice of normal control group were injected normal sodium to abdominal cavity.The inhibition rate of solid tumors in mice were calcul... |
| Keywords: | Mg2+-ATPasc |
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