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苯丙胺CPP重现实验中c-fos和p-CREB在大鼠纹状体表达的变化
引用本文:周媛,邢淑华.苯丙胺CPP重现实验中c-fos和p-CREB在大鼠纹状体表达的变化[J].中国药物依赖性杂志,2005,14(3):182-186.
作者姓名:周媛  邢淑华
作者单位:1. 江苏省苏州大学附属第二医院神经内科,苏州,215004
2. 江苏省徐州医学院药理学教研室,徐州,221002
基金项目:江苏省教育厅自然科学基金
摘    要:目的:研究苯丙胺致大鼠条件性位置偏爱及偏爱重现实验中纹状体c-fos和p-CREB的表达。方法:采用行为学和免疫组化技术。结果:(1)苯丙胺2·0mg·kg-1可使大鼠产生条件性位置偏爱效应,氢化可的松10mg·kg-1可使已消失的条件性位置偏爱效应重现;(2)苯丙胺可引起条件性位置偏爱大鼠纹状体的c-fos和p-CREB的高水平表达;(3)在氢化可的松诱发的条件性位置偏爱效应重现实验中,氢化可的松组在纹状体有c-fos和p-CREB的高水平表达。结论:c-fos和p-CREB蛋白表达参与了苯丙胺的条件性位置偏爱及重现效应的分子机制。

关 键 词:苯丙胺  条件性位置偏爱  纹状体  c-fos  cAMP反应元件结合蛋白
收稿时间:2004-11-19
修稿时间:2004年11月19

INCREASED EXPRESSION OF c- fos AND p- CREB IN RATS STRIATUM IN REOCCURRENCE OF CONDITIONED PLACE PREFERENCE INDUCED BY AMPHETAMINE
ZHOU Yuan,XING Shuhua.INCREASED EXPRESSION OF c- fos AND p- CREB IN RATS STRIATUM IN REOCCURRENCE OF CONDITIONED PLACE PREFERENCE INDUCED BY AMPHETAMINE[J].Chinese Journal of Drug Dependence,2005,14(3):182-186.
Authors:ZHOU Yuan  XING Shuhua
Abstract:Objective: To investigate the changes of c-fos and phospho-cAMP response element binding protein (p-CREB) in conditioned place preference (CPP) induced by amphetamine (Amp) and the reoccurrence of CPP rekindled by hydrocortisone in rats striatum. Methods: CPP model and the reoccurrence of CPP model were established and c-fos and p-CREB immunohistochemistry were used in both behavioral models.Results: (1)The rats developed CPP with amphetamine 2.0 mg·kg~ -1 and the vanished CPP could be rekindled by hydrocortisone 10 mg·kg~ -1;(2)The expression of c-fos and p-CREB increased in striatum in testing phase of CPP paradigm compared to pre-conditioning phase(P<0.01);(3)The expression of c-fos and p-CREB augmented in striatum in the reoccurrence of CPP provoked by hydrocortisone compared to saline control(P<0.01).Conclusion: c-fos and p-CREB protein participated in the mechanisms of both conditioned place preference and reoccurrence of CPP.
Keywords:c-fos
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