雷公藤不同样品对正常小鼠急性毒性实验研究

目的进行雷公藤不同样品对正常动物的急性毒性，为雷公藤的实验研究及临床安全使用提供实验依据。方法采用经典的急性毒性实验方法（LD。实验、MTD实验、MLD实验），给小鼠灌胃雷公藤不同样品（全组分、水提组分、雷公藤多甙片），观察其急性毒性症状谱，记录累积死亡数及小鼠体重变化。结果雷公藤全组分的MLD为8．0g·kg-1，相当于人日用量的22．4倍；水提组分的MLD为289．84g·kg^-1，相当于人日用量的811．6倍；雷公藤多甙片的LD。及95％可信限为166．34（157．60～175．51）mg·kg^-1，相当于人日用量的110．9倍，主要毒性症状、体征为怠动、俯卧、呼吸抑制，毒性发生时间较晚、持续时间长。结论雷公藤不同样品急性毒性大小顺序为雷公藤多甙片〉全组分〉水提组分，其体内毒性过程、毒性作用特点、毒性机制等尚待进一步研究。

Experimental Comparison Study on Mice＇s Acute Toxicity of Different Samples in Tripterygium Wilfordii

Objective The different samples to the acute toxicity in Tfipterygium wilfordii on mice were compared to provide the experimental data for the research and clinic of Trpterygqum wilfordii. Methods The all components, water extraction components from Tripterygium wilforclii and Tfipterygqum wilfordii polyglycoside（TWP） were prepared and compared in accordance with classical acute toxicity test methods. The toxic symptom was observed, and the death and the change of body weight were recorded. Results The MLD of all components and water extraction components are respectively 8.0 g＇kg~ and 289.84 g＇kg-1, which were respectively equal to 22.4 times and 811.6 times of 70 kg adult＇s daily dried medicinal herb expenses. The LDs0 and 95% confidence limit of TWP was 166.34 mgkg-1 （157.60--175.51 mg＇kg-1）, which was equal to 110.9 times of 70 kg adult＇s daily dried medicinal herb expenses. The main acute toxicity symptoms and signs were negligent action and prone fixed. The toxicity occurred late and lasted long time. Conclusion The acute toxicity of different components in Tripterygium wilfordii on mice is： TWP〉all components〉 water extraction components, but the process of toxicity in vivo, toxicity characteristics and toxicity mechanism are all not yet entirely clear, and they all need to be further studied.