植物内生真菌Talaromyces sp.的次级代谢产物研究

摘要：目的 对1株植物内生真菌Talaromyces sp.的次级代谢产物进行研究。方法 采用稻米培养基培养，通过正反相 硅胶柱层析、Sephadex LH-20凝胶柱层析、半制备反相高效液相色谱等技术对发酵提取物进行分离纯化，运用质谱、核磁 共振、圆二色谱等波谱技术进行结构鉴定，采用MTT测定化合物的细胞毒活性。结果 从该菌株中共分离鉴定11个已知化 合物，其中包括9个聚酮类化合物(1~9)和2个环肽类化合物(10和11)，分别为1-deoxyrubralactone(1)、alternariol(2)、alternariol 5-methyl ether(3)、altenuisol(4)、3-hydroxyalternariol 5-O-methyl ether(5)、(2'R,4'R,5'R)-altenuene(6)、(2'S,4'R,5'R)-isoaltenuene(7)、 (2'R,4'R,5'R)-altenuene-5'-acetoxyester(8)、7-hydroxy-3,5-dimethyl-isochromen-1-one(9)、腾毒素(10)和二氢腾毒素(11)。结论 化合 物1、化合物3~11为首次从该属菌株中分离，化合物2和3在100 μmol/L的浓度下对人膀胱癌J82细胞株具有抑制活性，抑制率分 别为56.9%和59.7%。

Study on the secondary metabolites of the endophytic fungus Talaromyces sp.

Abstract Objective To investigate the secondary metabolites of a plant endiphytic fungus Talaromyces sp. Methods The secondary metabolites were isolated from the crude extracts prepared from solid-substrate fermentation cultures by using various stationary and mobile phases, such as silica gel, reversed phase silica gel column chromatography, Sephadex LH-20 column chromatography, and semipreparative reversed-phase high performance liquid chromatography. Their structures were elucidated by nuclear magnetic resonance, mass spectrometry, and circular dichroism spectroscopy. The cytotoxic activities were assayed by MTT method. Results Eleven known compounds, including nine polyketides (1~9) and two cyclic peptides (10 and 11), were identified as 1-deoxyrubralactone (1), alternariol (2), alternariol 5-methyl ether (3), altenuisol (4), 3-hydroxyalternariol 5-O-methyl ether (5), (2'R,4'R,5'R)-altenuene (6), (2'S,4'R,5'R)-isoaltenuene (7), (2'R,4'R,5'R)-altenuene-5'-acetoxyester (8), 7-hydroxy-3,5-dim-ethyl-isochromen-1-one (9), toxin (10), and dihydrotoxin (11), respectively. Conclusion Compound 1, and compound 3~11 were isolated from the gennus Talaromyces for the first time. Compound 2 and 3 showed inhibitory against J82 cell line with inhibition rates of 56.9% and 59.7% at concentration of 100