海绵共附生放线菌Saccharopolyspora sp.nov中抗肿瘤活性成分的研究

羽毛山海绵（Mycale plumose）来源的糖多孢菌（Saccharopolyspora sp.nov SP2-10）具有诱导肿瘤细胞坏死的活性。本文对其发酵产物的活性部位乙酸乙酯层进行活性追踪分离．共得到7个化合物；利用理化性质和波谱学方法鉴定它们的结构分别为胆甾醇、邻苯二甲酸二异丁酯、4（2，4-二羟基苯甲酰氨基）苯甲酸、苯丙胺酸、脱氧鸟苷、鸟苷和N-乙酰酪胺I并初步评价上述化合物的抗肿瘤活性，结果表明，邻苯二甲酸二异丁酯显示强细胞毒活性。

Antitumor active components of Saccharopolyspora sp. novderived from Mycale plumose sponge

To explore the antitumor active components in ethyl acetate extract of Mycale plumose sponge-derived actinomycete Saccharopolyspora sp.nov by using solvent extraction,silica gel column,PHPLC and etc,seven compounds were isolated and purified.The separation procedure was guided by a bioassay using(tsFT210) cell lines.By means of physico-chemical properties and spectral analysis,their structures were determined as: cholesterine,1,2-benzendicarboxylic acid bis(2-methylpropyl) ester,4-(2,4-dihydroxybenzamido) benzoic acid,phenylalanine,deoxyguanosine,guanosine,N-acetyltyramine.The antitumor activity against(tsFT210) cell line of these compounds was assayed and 1,2-benzendicar-boxylic acid bis(2-methylpropyl) ester showed significant cytotoxic activity.