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| 测定司帕沙星间接竞争酶联免疫检测方法建立及应用 | |
| 引用本文： | 曾华金,刘兵,杨冉,雷利芳,屈凌波.测定司帕沙星间接竞争酶联免疫检测方法建立及应用[J].中国抗生素杂志,2011,36(7). |
| 作者姓名： | 曾华金 刘兵 杨冉 雷利芳 屈凌波 |
| 作者单位： | 1. 郑州大学药学院,郑州,450001 2. 郑州大学化学系,郑州,450001 3. 郑州大学化学系，郑州450001；河南工业大学化学化工学院，郑州450001 |
| 摘 要： | 目的 建立快速、灵敏的司帕沙星残留间接竞争酶联免疫检测方法。方法 采用混合酸酐法，将司帕沙星与牛血清白蛋白和卵清蛋白分别合成免疫抗原(sparfloxacin-BSA)和包被抗原(sparfloxacin-OVA)。通过背部多点免疫法免疫大白耳兔，制备了抗司帕沙星的特异性抗血清。结果 利用所获得的特异性抗血清，建立了测定司帕沙星的间接竞争酶联免疫检测方法(ciELISA)，其线性范围为5ng/mL～2μg/mL(R2=0.9942)，且与HPLC方法具有良好的相关性(R2=0.9918)。样品牛奶、大鼠血浆和尿液中的加样回收率分别为93.7～110.3％、105.6～113.3％和90.2～98.1％％。应用所建立的方法，对司帕沙星大鼠体内药物分析及药品中的含量进行了测定。结论 本研究所建立的检测司帕沙星残留的酶联免疫检测方法具有样品处理简单，灵敏度高及分析速度快等优点，非常适合于生物样品分析及药品残留的筛选工作。 |
| 关 键 词： | 司帕沙星 抗血清 ci-ELISA |
Establishment of a competitive inhibition enzyme-linked immunosorbent assay for determination of sparfloxacin and its applications |
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| Zeng Hua-jin,Liu Bing,Yang Ran,Lei Li-fang,Qu Ling-bo.Establishment of a competitive inhibition enzyme-linked immunosorbent assay for determination of sparfloxacin and its applications[J].Chinese Journal of Antibiotics,2011,36(7). | |
| Authors: | Zeng Hua-jin Liu Bing Yang Ran Lei Li-fang Qu Ling-bo |
| Institution: | Zeng Hua-jin1,Liu Bing2,Yang Ran2,Lei Li-fang2 and Qu Ling-bo2,3(1 School of Pharmaceutical Sciences,Zhengzhou University,Zhengzhou 450001,2 Department of Chemistry,3 School of Chemistry & Chemical Engineering,Henan University of Technology,Zhengzhou 450001) |
| Abstract: | Objective To establish a rapid and sensitive competitive inhibition enzyme-linked immunosorbent assay(ci-ELISA) for determination of sparfloxacin(SPFX).Methods Immunogen sparfloxacin-bovine serum albumin(SPFX-BSA) and coating antigen sparfloxacin-ovum albumin(SPFX-OVA) were prepared by a succinic anhydride method,respectively.By subcutaneous injection,the polyclonal antiserum was produced from big ear rabbits immunized with conjugates SPFX-BSA.Results As a reagent,the antiserum was also determined with a co... |
| Keywords: | Sparfloxacin Antiserum ci-ELISA |
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