| 哥斯达黎加链霉菌抗菌成分的活性跟踪分离与鉴定 |
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| 引用本文： | 郁蕾,戴好富,魏景,梅文莉,曾会才.哥斯达黎加链霉菌抗菌成分的活性跟踪分离与鉴定[J].中国抗生素杂志,2011,36(7). |
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| 作者姓名： | 郁蕾 戴好富 魏景 梅文莉 曾会才 |
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| 作者单位： | 1. 海南大学环境与植物保护学院，儋州571737；中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室，海口571101
2. 中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海口,571101 |
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| 基金项目： | 国家973计划前期研究专项 |
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| 摘 要： | 目的 分离、鉴定哥斯达黎加链霉菌(Stretomyces costaricanus)发酵液中的抗菌活性成分。方法 通过活性跟踪，采用硅胶柱层析等方法对哥斯达黎加链霉菌次生代谢产物进行分离纯化。采用光谱分析对活性成分进行结构解析。结果 哥斯达黎加链霉菌中的抗菌活性成分为放线菌素D。结论 首次发现放线菌素D对香蕉枯萎镰刀菌4号生理小种(Fusarium oxysporum f.sp.cubense Face 4，Focr4)，金黄色葡萄球菌(Staphyloccocus aureus)和耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus，MRSA)有较强的抑制活性。
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| 关 键 词： | 哥斯达黎加链霉菌 放线菌素D 抗菌活性 香蕉枯萎镰刀菌4号生理小种 |
Bioassay-guided isolation and identification of antibacterial component of Stretomyces costaricanus |
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| Yu Lei,Dai Hao-fu,Wei Jing,Mei Wen-li,Zeng Hui-cai.Bioassay-guided isolation and identification of antibacterial component of Stretomyces costaricanus[J].Chinese Journal of Antibiotics,2011,36(7). |
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| Authors: | Yu Lei Dai Hao-fu Wei Jing Mei Wen-li Zeng Hui-cai |
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| Institution: | Yu Lei1,Dai Hao-fu2,Wei Jing1,Mei Wen-li2 and Zeng Hui-cai2(1 College of Environment and Plant Protection,Hainan University,Danzhou 571737,2 Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101) |
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| Abstract: | Objective To isolate and identify antibacterial component from the fermentation broth of Stretomyces costaricanus.Methods Bioassay-guided silica gel column chromatography were used to separate the antibacterial component from the fermentation broth of Stretomyces costaricanus.The structure of the active component was elucidated by spectroscopic analyses.Results The antibacterial component of Stretomyces costaricanus was actinomycin D.Conclusion It's the first report that actinomycin D has strong inhibitory ... |
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| Keywords: | Stretomyces costaricanus Actinomycin D Antibacterial activity Fusarium oxysporum f sp cubense race 4 Focr4 |
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