Abstract: | Objective To understand the distribution of integrons in Proteus mirabilis, the types of variable region gene cassettes of positive strains, and their correlation with drug resistance phenotypes of host bacteria, so as to provide references for clinical treatment and nosocomial infection control. Methods Using PCR amplification and agarose gel electrophoresis, 150 strains of Proteus mirabilis isolated from clinical specimens from January 2016 to December 2018 were screened for the class 1,2 and 3 integrons. Sequencing analysis of variable regions of integron-positive strains and correlation analysis of drug resistance of host strains were carried out. Results There were 91 strains carrying 150 integrons in Proteus mirabilis. The positive rate was 60.7%, among which 30 strains were the first type of integron positive strains, accounting for 20%, 22 strains of the second type of integron positive strains, accounting for 14.7%, 39 strains carrying the first and the second type at the same time, accounting for 26%. No type 3 integrons were screened out. Among the 91 integron-positive strains, 86 mutant regions showed amplification product bands, and the other five strain regions showed no amplification products. The resistance gene cassettes carried by the variable region of the first type of integron positive strains were mainly AadA2 and DfrA32, and the variable region carrying the resistance class of the second type of integron positive strain was mainly DfrA1. The strain carrying the AadA2 in the variable region was celebrated. The resistance rate against gentamicin and tobramycin was significantly higher than that of the integron-negative strain (P<0.01), and the resistance rate of the strains with DfrA1 or DfrA32 to the compound sulfamethoxazole was also significantly higher than that of the strains without integrons (P<0.01). The resistance rates of 91 integron-positive strains to ampicillin/sulbactam, compound sulfamethoxazole, ciprofloxacin, gentamicin, ceftriaxone, tobramycin, and levofloxacin were significantly higher than those without integrons (P<0.01). Conclusion The clinically isolated Proteus mirabilis had a higher proportion of carrying integrons, and the drug resistance genes carried in the variable region were mainly genes encoding aminoglycosides and trimethoprim antibacterial drugs. The host bacteria of the integrons and the resulting resistance was highly |