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| 放线菌SPRI-70014杀草成分的研究 | |
| 引用本文： | 顾学斌,徐文平,叶高艺,张育雷,倪玮玮,陶黎明.放线菌SPRI-70014杀草成分的研究[J].中国抗生素杂志,2008,33(8). |
| 作者姓名： | 顾学斌 徐文平 叶高艺 张育雷 倪玮玮 陶黎明 |
| 作者单位： | 1. 上海市农药研究所,上海,200032 2. 上海南方农药研究中心,上海,201702 3. 上海市农药研究所,上海,200032;上海南方农药研究中心,上海,201702 |
| 基金项目： | 国家重点基础研究发展计划(973计划)，国家高技术研究发展计划(863计划) |
| 摘 要： | 一株来源于江西土壤代号为SPRI-70014的放线菌发酵产物具有很强的杀草活性,本文采用溶剂萃取、硅胶柱层析及制备HPLC等手段对该菌株发酵产物的活性组分进行了活性跟踪分离,得到纯度大于98%化合物纯品,通过理化性质及波谱学分析,鉴定其结构为2-2-(3,5二甲基-2-氧-环己基)-6-氧-四氢吡喃-4-基]-乙酰胺。并用除草剂生测标准化操作法(SOP)初步评价了该化合物除草活性。 |
| 关 键 词： | 放线菌 次级代谢物 乙酰胺 杀草活性 |
The herbicidal active component derived from soil actinomycete SPRI-70014 |
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| Gu Xue-bin,Xu Wen-ping,Ye Gao-yi,Zhang Yu-lei,Ni Wei-wei,Tao Li-ming.The herbicidal active component derived from soil actinomycete SPRI-70014[J].Chinese Journal of Antibiotics,2008,33(8). | |
| Authors: | Gu Xue-bin Xu Wen-ping Ye Gao-yi Zhang Yu-lei Ni Wei-wei Tao Li-ming |
| Abstract: | To explore the herbicidal active component in fermentation of a soil actinomycete SPRI-70014,a compound was isolated and purified by using solvent extraction,silica gel column chromatography and preparative HPLC.The separation procedure was traced by herbicidal activity.By means of physico-chemical properties and spectral analyses,the structure of the compound was determined as: 2-2-(3,5-Dimethyl-2-oxo-cyclohexyl)-6-oxo-tetrahydro-pyran-4yl]-acetamide.The herbicidal activity of the compound was assayed by SOP method. |
| Keywords: | Actinomycete Secondary metabolites Acetamide Herbicidal activity |
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