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| 海洋放线菌Y12-26代谢产物中bafilomycins分离纯化及结构鉴定 | |
| 引用本文： | 魏刚,苏超,张道敬,宗志友,斯聪聪,陶黎明.海洋放线菌Y12-26代谢产物中bafilomycins分离纯化及结构鉴定[J].中国抗生素杂志,2011,36(8). |
| 作者姓名： | 魏刚 苏超 张道敬 宗志友 斯聪聪 陶黎明 |
| 作者单位： | 1. 华东理工大学上海市化学生物学(芳香杂环)重点实验室,上海,200237 2. 华东理工大学生物反应器工程国家重点实验室,上海,200237 3. 上海师范大学生命与环境科学学院,上海,200234 4. 华东理工大学上海市化学生物学,芳香杂环,重点实验室,上海,200237 |
| 基金项目： | 国家高科技研究发展(863)计划 |
| 摘 要： | 目的 了解海洋放线菌Y12-26的次级代谢产物中对稻瘟病菌(Pyricularia oryzae)等植物病原真菌具有较好的拮抗活性的具体化合物.方法 以P.oryzae指示菌进行活性跟踪,对其代谢物进行分离提取,通过有机溶剂萃取、硅胶柱层析、TLC和HPLC制备等方法分离得到活性化合物,并通过UV、MS、NMR等方法进行结构鉴定.结果 从海洋放线菌Y12-26发酵物中分离得到了3个大环内酯类活性化合物Y12-26-A2a、A3a和A3b,确定Y12-26-A2a分子式为C<,35>H<,56>O<,8>,与bafilomycin D结构相同;Y12-26-A3a分子式为C<,35>H<,58>O<,9>,与bafilomycin A1结构相同;Y12-26-A3b为新结构化合物,分子式为C<,35>H<,58>O<,8>,命名为bafilomycin K.结论 化合物Y12-26-A2a、A3a和A3b对P.oryzae的MIC分别为125、16和31μg/mL. |
| 关 键 词： | 海洋放线菌 分离纯化 结构鉴定 bafilomycins |
Isolation, purification and structure identification of secondary metabolites produced by marine actinomycete Y12-26 |
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| Wei Gang,Su Chao,Zhang Dao-jing,Zong Zhi-you,Si Cong-cong,Tao Li-ming.Isolation, purification and structure identification of secondary metabolites produced by marine actinomycete Y12-26[J].Chinese Journal of Antibiotics,2011,36(8). | |
| Authors: | Wei Gang Su Chao Zhang Dao-jing Zong Zhi-you Si Cong-cong Tao Li-ming |
| Institution: | Wei Gang1,Su Chao1,Zhang Dao-jing2,Zong Zhi-you3,Si Cong-cong1 and Tao Li-ming1(1 Shanghai Key laboratory of Chemical Biology,East China University of Science and Technology,Shanghai 200237,2 State Key laboratory of Bioreactor Engineering,shanghai 200237,3 College of Life and Environment sciences,Shanghai Normal University,shanghai 200234) |
| Abstract: | Objective To know the active components in the secondary metabolites produced by marine actinomycete Y12-26 exhibited antagonistic activity against Pyricularia oryzae.Methods By bioassay-guided isolation and purification,the active antifungal compounds were isolated and purified from the culture extract of strain Y12-26 with solvent extract,silica gel chromatography,thin layer chromatography,and high performance liquid chromatography and other ways,and their structure were elucidation with the help of spect... |
| Keywords: | Marine actinomycete Isolation and purification Structure identification Bafilomycins |
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