| 气相色谱法分析方格星虫多糖的单糖组成 |
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| 引用本文： | 刘玉明,钱甜甜,李珂娴,何颖,沈先荣,江叔奇.气相色谱法分析方格星虫多糖的单糖组成[J].药物分析杂志,2012(8):1362-1364,1369. |
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| 作者姓名： | 刘玉明 钱甜甜 李珂娴 何颖 沈先荣 江叔奇 |
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| 作者单位： | 海军医学研究所;浙江中医药大学 |
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| 基金项目： | 国家科技重大专项新药创制项目(No.2008ZXJ09004-027) |
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| 摘 要： | 目的:研究方格星虫多糖的单糖组成。方法:采用3 mol.L-1三氟乙酸将方格星虫多糖水解成单糖,然后与盐酸羟胺进行衍生化反应,采用气相色谱法对其单糖组成进行分析。结果:方格星虫多糖的单糖组成主要为阿拉伯糖和葡萄糖,其质量分数分别为7.91%和79.32%。结论:此法简单、快速,重现性好,可用于方格星虫多糖的单糖组成分析。
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| 关 键 词： | 气相色谱法 方格星虫多糖 单糖组成分析 海洋星虫提取物 |
GC analysis of monosaccharides composition of polysaccharide in Sipunculus nudus Linnaeu |
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| LIU Yu-ming,QIAN Tian-tian,LI Ke-xian,HE Ying,SHEN Xian-rong,JIANG Shu-qi.GC analysis of monosaccharides composition of polysaccharide in Sipunculus nudus Linnaeu[J].Chinese Journal of Pharmaceutical Analysis,2012(8):1362-1364,1369. |
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| Authors: | LIU Yu-ming QIAN Tian-tian LI Ke-xian HE Ying SHEN Xian-rong JIANG Shu-qi |
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| Institution: | 1.Naval Medical Research Institute,Shanghai 200433,China;2.Zhejiang Traditional Medicine University,Hangzhou 310053,China) |
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| Abstract: | Objective:To determine the monosaccharide compositions of Sipunculus nudus polysaccharide.Methods:Polysaccharide samples were hydrolyzed with 3 mol·L-1 trifluoroacetic acid solution into monosaccharide,and then derivatized with hydroxylamine hydrochloride.The monosaccharide composition of Sipunculus nudus polysaccharide was analyzed by GC.Results:Sipunculus nudus polysaccharide was composed of arabinose and glucose with the mass fractions of 7.91% and 79.32%,respectively.Conclusion:The established method is simple,sensitive and accurate,which can be applied to the monosaccharide compositions analysis for Sipunculus nudus polysaccharide. |
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| Keywords: | gas chromatography Sipunculus nudus polysaccharide monosaccharide composition analysis Sipunculus nudus extraction |
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