| 抗恩诺沙星抗血清的制备及其ELISA检测 |
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| 引用本文： | 刘兵,曾华金,杨冉,雷利芳,屈凌波.抗恩诺沙星抗血清的制备及其ELISA检测[J].药物分析杂志,2011(7). |
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| 作者姓名： | 刘兵 曾华金 杨冉 雷利芳 屈凌波 |
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| 作者单位： | 郑州大学化学系;郑州大学药学院;河南工业大学化学化工学院; |
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| 摘 要： | 目的:建立快速、灵敏的恩诺沙星残留酶联免疫检测方法(ELISA)。方法:采用混合酸酐法,将恩诺沙星与牛血清白蛋白和卵清蛋白分别合成免疫抗原(Enrofloxac in-BSA)和包被抗原(Enrofloxac in-OVA)。通过背部多点免疫法免疫日本大白耳兔,制备抗恩诺沙星的特异性抗血清。结果:利用所获得的特异性抗血清,建立的恩诺沙星ELISA检测方法,其线性范围为5 ng.mL-1～2.5μg.mL-1(R2=0.9935),且与HPLC方法具有良好的相关性(R2=0.9892,n=9)。牛奶、大鼠血浆和尿液中的加样回收率分别为98.4%～105.8%,91.7%～101.2%,97.0%～110.3%。运用所建立的方法,对大鼠体内血浆及药品中恩诺沙星的含量进行了测定。结论:本研究所建立的检测恩诺沙星的ELISA方法具有样品前处理简单,灵敏度高及分析速度快等优点,适合用于恩诺沙星的体内分析及动物性食品中恩诺沙星的残留分析。
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| 关 键 词： | 恩诺沙星 乙基环丙沙星 抗血清 酶联免疫检测法 牛血清白蛋白 免疫抗原 卵清蛋白 包被抗原 食品 药物残留 快速分析 |
Preparation of enrofloxacin antiserum and its determination by enzyme-linked immunosorbent assay(ELISA) |
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| LIU Bing,ZENG Hua-jin,YANG Ran,LEI Li-fang,QU Ling-bo.Preparation of enrofloxacin antiserum and its determination by enzyme-linked immunosorbent assay(ELISA)[J].Chinese Journal of Pharmaceutical Analysis,2011(7). |
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| Authors: | LIU Bing ZENG Hua-jin YANG Ran LEI Li-fang QU Ling-bo |
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| Institution: | LIU Bing1,ZENG Hua-jin2,YANG Ran1,LEI Li-fang1,QU Ling-bo1,3(1.Department of Chemistry,Zhengzhou University,Zhengzhou 450001,China,2.School of Pharmaceutical Sciences,3.Chemistry and Chemical Engineering,Industry University of Henan,China) |
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| Abstract: | Objective:To develop a rapid and sensitive enzyme-linked immunosorbent assay(ELISA) for determination of enrofloxacin(ENR).Method:Immunogen enrofloxacin-bovine serum albumin(Enrofloxacin-BSA) and coating antigen enrofloxcin-ovum albumin(Enrofloxacin-OVA) were prepared by a succinic anhydride method.By subcutaneous injection,the polyclonal antiserum was produced from big ear rabbits immunized with conjugates ENR-BSA.Result:After the assay procedure was optimized,the standard curve of ENR was established and ... |
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| Keywords: | ethyl ciprofloxacin enrofloxacin antiserum ELISA bull serum albumin(BSA) immune antigen ovalburmin(OVA) envelope antigen food drug residue rapid analysis |
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