| 高效液相色谱法建立银耳多糖特定图谱的研究 |
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| 引用本文： | 陈英红,姜瑞芝,罗浩铭,王颖,高其品.高效液相色谱法建立银耳多糖特定图谱的研究[J].药物分析杂志,2012(1):136-139. |
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| 作者姓名： | 陈英红 姜瑞芝 罗浩铭 王颖 高其品 |
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| 作者单位： | 吉林省中医药科学院;长春中医药大学 |
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| 基金项目： | 国家十一五科技重大专项(No:2009ZX09103-333);吉林省科技发展计划项目(No:20100907) |
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| 摘 要： | 目的:建立银耳多糖特定图谱。方法:通过对银耳多糖PMP柱前衍生化,采用高效液相色谱法进行糖组成分析。结果:银耳多糖特定图谱由6个共有峰组成,为甘露糖、葡萄糖醛酸、葡萄糖、木糖、岩藻糖以及1个未鉴定出的单糖。结论:高效液相色谱法建立多糖的特定图谱,其操作方法简便,分离度高,重复性及稳定性良好,该结果可有效控制银耳多糖的内在质量,同时可作为酸性杂多糖糖组成的测定方法。
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| 关 键 词： | 银耳多糖 甘露糖 葡萄糖醛酸 葡萄糖 木糖 岩藻糖 单糖 指纹图谱 柱前衍生化 高效液相色谱法 |
Studies on establishing specific chromatogram of Tremella polysaccharides by HPLC |
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| CHEN Ying-hong,JIANG Rui-zhi,LUO Hao-ming,WANG Ying,GAO Qi-pin.Studies on establishing specific chromatogram of Tremella polysaccharides by HPLC[J].Chinese Journal of Pharmaceutical Analysis,2012(1):136-139. |
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| Authors: | CHEN Ying-hong JIANG Rui-zhi LUO Hao-ming WANG Ying GAO Qi-pin |
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| Institution: | 1.Jilin Academy of Traditional Chinese Medicine and Material Medical Sciences,Changchun 130012,China; 2.Changchun University of Chinese Traditional Medicine,Changchun 130117,China) |
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| Abstract: | Objective:To establish specific chromatogram of Tremella polysaccharides(TP).Methods: The monosaccharide composition of Tremella polysaccharides was determined by HPLC after preparing pre-column derivatization as PMP derivant.Results:There were 6 peaks from the fingerprint which were mannose,glucose acid,glucose,xylose,fucose and an unidentifiable monosaccharide respectively.Conclusion: The HPLC method establishing specific chromatogram is a simple,rapid,sensitive and convenient,which can be applied to the quality control of Tremella polysaccharides.And it also can be used to determine monosaccharide composition of acidic heteropolysaccharides. |
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| Keywords: | Tremella polysaccharides mannose glucose acid glucose xylose fucose monosaccharide fingerprint chromatogram pre-column derivatization HPLC |
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