| 高效液相色谱法测定何首乌和夜交藤中蒽醌类成分的含量 |
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| 引用本文： | 大岛俊幸,王珂.高效液相色谱法测定何首乌和夜交藤中蒽醌类成分的含量[J].药物分析杂志,1996,16(4):219-222. |
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| 作者姓名： | 大岛俊幸 王珂 |
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| 作者单位： | 日本大正制药株式会社综合研究所(王珂)，中国医学科学院 中国协和医科大学药物研究所(童玉懿) |
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| 摘 要： | 建立了何首乌中的主要有效成分大黄素、大黄素甲醚、大黄酚及大黄酸等蒽醌类的反相高效液相色谱法。以ＴＳＤ－ｇｅｌ８０Ｔｓ为固定相，甲醇－水－磷酸（７２０：２８０：１）为流动相。样品用７０％甲醇提取浓缩后用氯仿萃取，分离出游离型和结合型蒽醌的定量用试液。本法精确、可靠。用此方法测定了不同产地的９种何首乌和４种夜交藤样品。
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| 关 键 词： | 何首乌 夜交藤 蒽醌类 高效液相色谱法 |
The Analysis of Anthraquinones in Polygonum multiflorum by HPLC |
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| Toshiyuki Oshima, Fusayoshi Hirayama, Fumitaka Saitou and Kazuhiko Sagara.The Analysis of Anthraquinones in Polygonum multiflorum by HPLC[J].Chinese Journal of Pharmaceutical Analysis,1996,16(4):219-222. |
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| Authors: | Toshiyuki Oshima Fusayoshi Hirayama Fumitaka Saitou and Kazuhiko Sagara |
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| Abstract: | High performance liquid chromatographic method was developed for the determination of free and glycosidic anthraquinones, namely rhein, emodin, chrysophanol and physcion and their glycosides, in Polygonum multiflorum. A reversed-phase system consisting of a chemically bonded ODS silica gel column with a mixture of methanol-water-phosphoric acid (720 : 280 : 1) as the mobile phase was used. The sample was extracted by supersonic with 70%methanol and concentrated, then partitioned with chloroform and water to separate free and glycosidic anthraquinone fractions. Furthermore, glycosidic anthraquinone fraction was hydrolysed to free form. Market samples were determined by this method. Emodin, physcion and their glycosides were found in Polygonum multiflorum, but the other anthraquinones were not detected. |
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| Keywords: | Polygonum multiflorum Caulis polygoni anthraquinones rhein emodin chrysophanol physcion HPI C |
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