| 枸骨叶中3类三萜皂苷类成分的ESI-MS裂解规律及其快速识别 |
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| 引用本文： | 张加余,姚志容,张凡,屠鹏飞.枸骨叶中3类三萜皂苷类成分的ESI-MS裂解规律及其快速识别[J].药物分析杂志,2012(9):1578-1582. |
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| 作者姓名： | 张加余 姚志容 张凡 屠鹏飞 |
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| 作者单位： | 北京大学药学院天然药物及仿生药物国家重点实验室;北京中医药大学中药学院 |
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| 基金项目： | 国家自然科学基金(30672608);国家重大新药创制专项(2009ZX311-004) |
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| 摘 要： | 目的:研究枸骨叶中3类不同母核结构的三萜皂苷的ESI-MS裂解规律及其快速识别方法。方法:采用HPLC-ESI-MS技术研究3类三萜皂苷单体的ESI-MS(+)裂解规律,并采用该方法对药材中所含的3类成分进行快速识别。结果:3类三萜皂苷具有不同的ESI-MS(+)裂解特征;利用这些特征从枸骨叶提取物中快速筛选了29个三萜皂苷,其中10个可通过与对照品比对被准确鉴定。结论:利用HPLC-ESI-MS技术可以从复杂体系中快速筛选三萜皂苷类成分,为有目标的天然产物分离提供有效的方法。
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| 关 键 词： | 三萜皂苷 枸骨叶(苦丁茶) 液质联用 ESI-MS裂解规律 化学筛选 结构特征 物质基础 在线快速鉴定 |
ESI-MS characterization and rapid identification of three types of triterpenoid saponins in Ilex cornuta |
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| ZHANG Jia-yu,YAO Zhi-rong,ZHANG Fan,TU Peng-fei.ESI-MS characterization and rapid identification of three types of triterpenoid saponins in Ilex cornuta[J].Chinese Journal of Pharmaceutical Analysis,2012(9):1578-1582. |
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| Authors: | ZHANG Jia-yu YAO Zhi-rong ZHANG Fan TU Peng-fei |
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| Institution: | 1 (1.State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University Health Science Center, Beijing 100191,China;2.School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102,China) |
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| Abstract: | Objective:To investigate the ESI-MS characterization and rapid identification of three types of triterpenoid saponins with different parent nucleuses in Ilex cornuta.Methods: HPLC-ESI-MS method was used to investigate the ESI-MS(+) characteristics of those triterpenoid saponins,which were adopted to indentify three types of triterpenoid saponins in Ilex cornuta.Results: Three types of triterpenoid saponins have different ESI-MS(+) characteristics,by which 29 triterpenoid saponins were rapidly identified from the extract of Ilex cornuta.Among them,10 compounds were accurately identified by comparison with reference substances.Conclusions:HPLC-ESI-MS has been provided not only as a new thread and method for rapidly identification of triterpenoid saponins from complex system,but also an effective method for target separation of constituents in natural drugs. |
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| Keywords: | triterpenoid saponins Ilex cornuta(Kudingcha/broadleaf holly leaf) HPLC-MS ESI-MS characterization chemical screening structural feature material base online rapid identification |
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