| 反相高效液相色谱法测定清炎颗粒中绿原酸的含量 |
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| 引用本文： | 崔岚,祝德秋,王晓珉,罗启剑,曹惠明.反相高效液相色谱法测定清炎颗粒中绿原酸的含量[J].中国药房,2004,15(6):368-369. |
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| 作者姓名： | 崔岚 祝德秋 王晓珉 罗启剑 曹惠明 |
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| 作者单位： | 上海第二医科大学附属仁济医院临床药学室,上海市,200001 |
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| 摘 要： | 目的 :建立以反相高效液相色谱法测定清炎颗粒中绿原酸含量的方法。方法 :采用HypersilBDSC18 色谱柱 ,以甲醇 -0 4 %磷酸溶液 (20∶80)为流动相 ,检测波长为327nm。结果 :绿原酸在进样量174～1392ng之间与峰面积积分值呈良好的线性关系(r=0 9999) ,平均回收率为100 10 %,RSD=1 60 %(n=5)。结论 :本方法灵敏、简便、重现性好 ,可有效地控制清炎颗粒质量。
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| 关 键 词： | 清炎颗粒 绿原酸 反相高效液相色谱法 含量测定 |
| 文章编号： | 1001-0408(2004)06-0368-02 |
| 修稿时间： | 2003年7月8日 |
Determination of Chlorogenic Acid in Qingyan Granules by RP-HPLC |
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| CUI Lan,ZHU Deqiu,WANG Xiaomin,LUO Qijian,CAO Huiming.Determination of Chlorogenic Acid in Qingyan Granules by RP-HPLC[J].China Pharmacy,2004,15(6):368-369. |
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| Authors: | CUI Lan ZHU Deqiu WANG Xiaomin LUO Qijian CAO Huiming |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for the determination of chlorogenic acid in Qingyan granules.METHODS:Waters Breeze System was used with a Hypersil BDS C18 column(5μm,4.6mm×150mm).The mobile phase was composed of methanol and 0.4%(V/V)H3PO4 water solution(20∶80,V/V),flow rate:1.0ml/min;wavelength of UV detector:327nm,column temperature 20℃.RESULTS:The calibration curve of chlorogenic acid was linear within the range of (174～1 392)ng(r=0.9 999).The average recovery was 100.10%,RSD=1.60%(n=5).CONCLUSION:This method is simple,sensitive,as well as reliable,and is available to quality control. |
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| Keywords: | Qingyan granule chlorogenic acid RP-HPLC content determination |
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