| 灵芝多糖对小鼠T细胞蛋白激酶A和蛋激酶C活性的影响 |
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| 引用本文： | 李明春,雷林生,王庆彪,梁东升,许自明,杨淑琴,孙莉莎.灵芝多糖对小鼠T细胞蛋白激酶A和蛋激酶C活性的影响[J].中国药房,2001,12(2):78-79. |
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| 作者姓名： | 李明春 雷林生 王庆彪 梁东升 许自明 杨淑琴 孙莉莎 |
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| 作者单位： | 1. 解放军第401医院药剂科,
2. 第一军医大学药理教研室, |
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| 基金项目： | 国家自然科学基金资助项目(39400165) |
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| 摘 要： | :目的 :观察灵芝多糖体外对小鼠T细胞蛋白激酶A (PKA)和蛋白激酶C(PKC)活性是否有影响。方法 :采用反相离子对高效液相色谱法测定PKA和PKC活力。结果 :灵芝多糖GLB7 能引起T细胞中PKA和PKC活性明显增强并具有剂量依赖性 ,达峰时间分别为5min和20min ,于20min及1h分别恢复到基础水平 ;GLB7 还可引起T细胞中PKC发生质膜转位 ,并拮抗Stau rosporine对T细胞中PKC的抑制作用。结果 :灵芝多糖的免疫增强和抗肿瘤作用与其增强PKA和PKC活性有关
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| 关 键 词： | 灵芝 多糖 T细胞 蛋白激酶A 蛋白激酶C |
| 文章编号： | 1001-0408(2001)02-0078-02 |
| 修稿时间： | 2000年7月10日 |
Effects of Ganoderma
Polysacchrides on PKC and PKA Activities in Murine T Cells |
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| Li Mingchun,LIANG Dongsheng,XU Ziming,LEI Linsheng,WANG Qingbiao,YANG Shuqin,SUN Lisha.Effects of Ganoderma
Polysacchrides on PKC and PKA Activities in Murine T Cells[J].China Pharmacy,2001,12(2):78-79. |
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| Authors: | Li Mingchun LIANG Dongsheng XU Ziming LEI Linsheng WANG Qingbiao YANG Shuqin SUN Lisha |
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| Abstract: | AIM：To investigated the effects of Ganoderma polysaccthride(GLB7)on protein kinase A(PKA) and protein kinase C(PKC) activitives in murine T cells.METHODS：A new ion-pair reversed-phase high liquid chromatography method was used to determinate the activities of PKA and PKC in T cells.RESULTS：GLB7 could markedly increase the activities of PKA and PKC in murine T cells in a dose-dependent manner.The peak time was at 5 min and 20 min and the activities of PKA and PKC returned to basic level at 20 min and 1.5h respectively.GLB7 could induce translocation of PKC and antagonize the inhibition effect of staurosporine(10μ mol· L-1) on PKC in T cells.CONCLUSION：The immunopotentiating and antitumour effects of Ganoderma polysacchride may be associated with its activation on PKA and PKC in murine T cells. |
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| Keywords: | Ganoderma Lucidum polysaccharide T cell protein kinase A protein kinase C |
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