黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用

目的:研究黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用。方法:100只雌性ICR小鼠随机分为正常对照(等容生理盐水)组、黄芪多糖对照(80 mg/kg)组、黄芪甲苷对照(80 mg/kg)组、模型(等容生理盐水)组、黄芪多糖(80 mg/kg)组、黄芪甲苷(80 mg/kg)组与联合用药①、②、③、④(黄芪多糖80、40、20、80 mg/kg+黄芪甲苷20、40、80、80 mg/kg)组。灌胃给药,每天1次,连续14 d。末次给药后小鼠接受60Coγ射线(剂量:5 cy)一次性全身辐射以复制辐射损伤模型。测定小鼠30 d存活率和死亡小鼠平均存活时间;肝脏HE染色光镜下观察小鼠肝组织形态学;测定小鼠外周血白细胞数、骨髓细胞DNA含量、血清超氧化物歧化酶(SOD)活性。结果:与正常对照组比较,模型组小鼠30 d存活率降低,死亡小鼠平均存活时间缩短;小鼠肝细胞广泛肿胀,出现大滴性脂肪变、气球样变,肝脏细胞点状坏死严重,炎性因子大面积浸润组织;外周白细胞计数减少;骨髓细胞DNA含量减少;血清SOD活性减弱,差异具有统计学意义(P<0.01)。与模型组比较,联合用药①、②、③、④组小鼠30 d存活率升高,死亡小鼠平均存活时间延长,外周白细胞计数增加,骨髓细胞DNA含量增加,小鼠肝细胞形态学明显改善;联合用药①、②、③组小鼠血清中SOD活性增强,差异具有统计学意义(P<0.01)。结论:黄芪多糖与黄芪甲苷配伍是通过多靶点发挥作用,其中黄芪多糖与黄芪甲苷质量比为4∶1时配伍效果最佳。

Protective Effects of Astragali Radix Polysaccharides Combined with Astragaloside IV against Radiation Injury Model Mice

OBJECTIVE： To study the protective effects of Astragali Radix polysaccharides and astragaloside IV on radiation model mice. METHODS： 100 female ICR mice were randomized into normal control group（constant volume of normal saline）, Astragali Radix polysaccharides control group （80 mg/kg）, astragaloside IV control group （80 mg/kg）, model group （constant volume of normal saline）, Astragali Radix polysaccharides group （80 mg/kg）, astragaloside IV group （80 mg/kg） and drug combination group I （Astragali Radix polysaccharides 80 mg/kg＋astragaloside IV 20 mg/kg）, drug combination group I1 （Astragali Radixpolysaccharides 40 mg/kg＋astragaloside IV 40 mg/kg） and drug combination group IH （Astragali Radix polysaccharides 20 mg/kg＋ astragaloside IV 80 mg/kg） and drug combination group IV （Astragali Radix polysaccharides 80 mg/kg＋astragaloside IV 80 mg/kg）. They were given relevant medicine intragastrically once a day for consecutive 14 days. Radiation injury model was induced by disposable full-body exposure 60Coγ ray （5 cy）after last administration. Survival rate of mice within 30 d and average survival duration of dead mice were determined. Hepatocyte morphology of mice was observed by HE staining. The number of peripheral blood leucocyte, DNA content of marrow cells and the activities of SOD in serum were determined. RESULTS： Compared with normal control group, the survival rate of model group within 30 d and average survival duration were decreased significantly. Hepatic cell swelling, large lipid drops fat denaturation and ballooning degeneration were observed, and severe spotty necrosis of hepatic cell and large area infiltration of inflammatory factor were also found. The peripheral blood leucocyte count, DNA content of marrow cells and the activities of SOD in serum were decreased significantly （P〈0.01）. Compared with model group, 30 d survival rate of drug combination groups I , II , III and IV were increased significantly, and average survival duration of dead mice, peripheral blood leucocyte count and DNA content of marrow cells were increased significantly; hepatic cell morphology of mice was improved significantly. The activities of SOD in serum were increased significantly in drug combination group I , II and IU （P〈 0.01）. CONCLUSIONS： The compatibility of Astragali Radix polysaccharides and astragaloside IV can alleviate damage induced by irradiation by multi-target. Astragali Radix polysaccharides to astragaloside IV （mass ratio 4： 1） are the most effective.