| 槲皮素代谢产物定性检出条件研究 |
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| 引用本文： | 任守忠,陈峰,刘明生,符乃光,魏娜,张俊清.槲皮素代谢产物定性检出条件研究[J].中国药房,2009(3):171-173. |
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| 作者姓名： | 任守忠 陈峰 刘明生 符乃光 魏娜 张俊清 |
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| 作者单位： | 海南医学院药学系/海南省热带药用植物研究与开发重点实验室,海口市571101 |
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| 基金项目： | 海南省自然科学基金资助项目（30627） |
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| 摘 要： | 目的:研究给予大鼠槲皮素后血浆中代谢产物的定性检出条件。方法:给予大鼠槲皮素单体(100mg.kg-1)后腹腔静脉取血,血浆样品经2mol.L-1盐酸(甲醇-水溶液)水解,采用高效液相色谱法对血浆样品中代谢产物进行定性分析。结果:大鼠给药后3h内取血、血浆样品经盐酸水解处理4h,可稳定检测槲皮素和异鼠李素,样品在—20℃条件下贮存稳定。结论:血浆样品采集时间、盐酸水解时间及贮存方式等不同均可影响代谢产物的有效检出。
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| 关 键 词： | 高效液相色谱法 槲皮素 异鼠李素 定性检出 代谢产物 |
Conditions of Qualitative Determination of Metabolite of Quercetin |
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| REIN- Shou-zhong,CHEN Feng,LIU Ming-sheng,FU Nai-guang,WEI Na,ZHANG Jun-qing.Conditions of Qualitative Determination of Metabolite of Quercetin[J].China Pharmacy,2009(3):171-173. |
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| Authors: | REIN- Shou-zhong CHEN Feng LIU Ming-sheng FU Nai-guang WEI Na ZHANG Jun-qing |
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| Institution: | (Dept. of Pharmacy, Hainan Medical College, Hainan Provincial Key Laboratory of R＆D of Tropical Herbs, Haikou 571101, China) |
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| Abstract: | OBJECTIVE：To conduct a qualitative determination of the metabolite of quercetin in rat plasma.METHODS： The plasma sample was taken from rats after an oral administration of monomer of quercetin（100mg·kg^-1） and were hydrolyzed by 2 mol · L^-1 hydrochloric acid（methanol-water solution） .The metabolite of quercetin in rat plasma was qualitatively determined by HPLC. RESULTS： Quercetin and isorhamnetin could be stably determined if serum sample was taken within 3 hours after oral administration of monomer of quercetin and hydrolyzed by hydrochloric acid for 4 hours. The serum sample was stable stored in -20℃; refrigerator.CONCLUSION： The sampling time, hydrolization time and the storage mode etc of the serum sample have impacts on the effective qualitative determination of the metabolite in quercetin. |
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| Keywords: | HPLC Quercetin Isorhamnetin Qualitative determination Metabolites |
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