| RP-HPLC法测定吉非替尼分散片的含量 |
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| 引用本文： | 刘妍,林华庆,余楚钦,侯悦翰.RP-HPLC法测定吉非替尼分散片的含量[J].中国药房,2012(13):1225-1226. |
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| 作者姓名： | 刘妍 林华庆 余楚钦 侯悦翰 |
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| 作者单位： | 广东药学院药物研究所,广东省药物新剂型重点实验室,广州510006 |
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| 摘 要： | 目的:建立测定吉非替尼分散片中主药含量的方法。方法:采用反相高效液相色谱法。色谱柱为Diamonsil C18(2),流动相为乙腈-0.015%三氟乙酸(26:74,三乙胺调pH值为3.9),流速为1.0mL.min-1,检测波长为344nm,柱温为30℃。结果:吉非替尼检测浓度线性范围为0.08～6.05μg.mL-1(r=0.9998);平均回收率为100.55%,RSD=1.28%(n=9)。结论:该方法操作简便、快速、重复性好,可用于吉非替尼分散片的含量测定。
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| 关 键 词： | 吉非替尼分散片 反相高效液相色谱法 含量测定 |
Content Determination of Gefitinib Dispersible Tablets by RP-HPLC |
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| LIU Yan,LIN Hua-qing,YU Chu-qin,HOU Yue-han.Content Determination of Gefitinib Dispersible Tablets by RP-HPLC[J].China Pharmacy,2012(13):1225-1226. |
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| Authors: | LIU Yan LIN Hua-qing YU Chu-qin HOU Yue-han |
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| Institution: | (Guangdong Provincial Key Lab of Advanced Drug Deliv- ery, Institute of Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China) |
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| Abstract: | OBJECTIVE： To establish the method for the content determination of main component in Gefitinib dispersible tablet. METHODS： RP-HPLC method was adopted. The separation was achieved by Diamonsil C18 （2） column using acetoni- trile-0.015% trifluoroacetic acid （26：74, pH adjusted to 3.9 with triethanolamine） as mobile phase at the flow rate of 1.0 mL.min-t. The detection wavelength was set at 344 nm and the column temperature was 30 ℃. RESULTS： The linear range of gefitinib was 0.08-6.05 μg.mL-1 （r=0.999 8） with an average recovery of 100.55% （RSD=1.28%, n=9）. CONCLUSION： The method is simple, rapid and reproducible, and it is suitable for the content determination in Gefitinib dispersible tablet. |
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| Keywords: | Gefitinib dispersible tablet RP-HPLC Content determination |
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