异甘草酸镁对D-氨基半乳糖急性肝损伤模型小鼠的保护作用研究

目的研究异甘草酸镁对D-氨基半乳糖急性肝损伤模型小鼠的保护作用。方法以D-氨基半乳糖建立小鼠急性肝损伤模型,分别设正常对照组、病理模型组、异甘草酸镁高、低剂量组以及美能组、易善复组、凯西莱组,测定各组小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬酸氨基转移酶(AST)活性及肝组织中丙二醛(MDA)、黄嘌呤氧化酶(XOD)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、总抗氧化能力(T-AOC)、一氧化氮(NO)、一氧化氮合成酶(NOS)、诱生型一氧化氮合酶(iNOS)含量。另以HE染色,采用光镜对各组小鼠肝脏作病理检查。结果异甘草酸镁可明显降低ALT、AST的活性(P<0.01)及肝组织中MDA、XOD、NO、NOS、iNOS的含量;升高肝组织中SOD、GSH-PX、T-AOC的含量。光镜下可观察到异甘草酸镁能减轻小鼠肝脏坏死性病理改变。结论异甘草酸镁对D-氨基半乳糖诱导的小鼠急性肝损伤具有良好的保护作用。

Protective Effect of Magnesium Isoglycyrrhizina on Acute Hepatic Injury in Mice

OBJECTIVE: To investigate the protective effect of MgIG on acute hepatic injury in mice induced by D-GalN. METHODS: The acute hepatic injury model of mice was induced by D-GalN. The mice were divided into control group, patho-model group, MgIG high and low dose group,compound glycyrrhizin group,Essentiale N group and Tiopronin group. The changes of activity of ALT, AST in serum and MDA, SOD, XOD,GSH-PX,T-AOC, NO, NOS, iNOS content in liver tissue were measured. Then by HE staining the pathologic change of liver tissue was examined under light microscope. RESULTS: MgIG could decrease(P<0.01) the activities of ALT, AST in serum and MDA, XOD,NO,NOS, iNOS content in liver tissue; and increase the SOD, GSH-PX, T-AOC content in liver tissue. It was observed that MgIG could significantly alleviate the necrotic pathologic change of liver tissue. CONCLUSION: MgIG has protective function against acute liver injury in mice induced by D-GalN.