几种中药有效成分联合应用对小鼠肉瘤细胞株S180的作用

目的:探讨中药有效成分联用时的相互作用。方法:采用MTT法测定几种中药有效成分(高良姜素、厚朴酚、姜黄素、喜树碱、苦参碱、人参皂苷Rg1单药)单用和联用对肉瘤细胞株的增殖抑制率,用抑制浓度的分数之和(SFIC)及联用表观抑制率(OI)分析方法评价协同用药的效果。结果:喜树碱与苦参碱、喜树碱与高良姜素1∶1(质量比)联用表现出较好的协同作用;喜树碱与苦参碱联用半数抑制浓度(IC50)为2.918μg.mL-1;喜树碱与高良姜素联用IC50为56.49μg.mL-1。所有三者联用以及其它两两联用都表现出拮抗作用。结论:中药有效成分联合使用时,相互之间存在着协同与拮抗作用。

Interaction among Several Effective Chemical Compositions in Chinese Material Medicine on Cell Line S180

OBJECTIVE:To observe the effects of Galangin,Magnolol,Curcumin,Camptothecine,Matrine and Ginsenoside Rg1 on cell line S180 and the interaction of two-drug combination and three-drug combination.METHODS:The inhibitions of effective chemical compositions(ECCs)in Chinese material medicine on S180 cells were measured by MTT assay.The principle of the interaction between various ECCs and the possibility of drug combination were discussed.The sum of fractional inhibitory concentration(SFIC)and ostensible inhibition(OI)methods were used for evaluation of the interaction between various ECCs.RESULTS:Both the combination of Camptothecine and Matrine,and the combination of Camptothecine and Galangin by 1:1 proportion showed obvious synergism.IC50 of the combination of Camptothecine and Matrine is 2.918μ g· mL-1,IC50 of the combination of Camptothecine and Galangin is 56.49μ g· mL-1.Combination of all three compounds and other combination of two compounds showed antagonism.CONCLUSION:Synergistic and antagonistic effects exist in combinations of ECCs in Chinese material medicine.